Expression of Recombinant Cold-Adapted (Hemo)Globins from Marine Bacteria.

Giordano, Daniela; Verde, Cinzia

Giordano, Daniela; Verde, Cinzia.

Afiliación

Giordano D; Institute of Biosciences and BioResources (IBBR), CNR, Naples, Italy. daniela.giordano@ibbr.cnr.it.
Verde C; Department of Marine Biotechnology, Stazione Zoologica Anton Dohrn (SZN), Villa Comunale, Naples, Italy. daniela.giordano@ibbr.cnr.it.

Methods Mol Biol ; 2498: 283-292, 2022.

Article en En | MEDLINE | ID: mdl-35727550

RESUMEN

The production of recombinant proteins in bacteria made possible to obtain large quantities of proteins essential for basic and applied research. Escherichia coli remains one of the organisms of choice for recombinant proteins because of its ability to grow at high density and availability of a vast catalog of cloning vectors and mutant host strains. Here, we describe the protocols for the expression of cold-adapted (hemo)globins in Escherichia coli.

Asunto(s)

Escherichia coli; Globinas; Clonación Molecular; Escherichia coli/genética; Escherichia coli/metabolismo; Vectores Genéticos/genética; Globinas/metabolismo; Proteínas Recombinantes/metabolismo

Palabras clave

Bacterial hemoprotein; Cold adaptation; Escherichia coli; Expression plasmid; Recombinant-protein expression; UVvisible absorption spectrum

Texto completo

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Globinas / Escherichia coli Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2022 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos

Texto completo

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Buscar en Google