Ti Ions Induce IL-1ß Release by Activation of the NLRP3 Inflammasome in a Human Macrophage Cell Line.

Pettersson, Mattias; Almlin, Sanna; Romanos, Georgios E; Johansson, Anders

Pettersson, Mattias; Almlin, Sanna; Romanos, Georgios E; Johansson, Anders.

Afiliación

Pettersson M; Division of Prosthetic Dentistry, Department of Odontology, Faculty of Medicine, Umeå University, SE-901 87, Umeå, Sweden. mattias.p.pettersson@umu.se.
Almlin S; Division of Prosthetic Dentistry, Department of Odontology, Faculty of Medicine, Umeå University, SE-901 87, Umeå, Sweden.
Romanos GE; Department of Periodontology, School of Dental Medicine, Stony Brook University, Stony Brook, NY, 11794, USA.
Johansson A; Molecular Periodontology, Department of Odontology, Faculty of Medicine, Umeå University, Umeå, Sweden.

Inflammation ; 45(5): 2027-2037, 2022 Oct.

Article en En | MEDLINE | ID: mdl-35726039

RESUMEN

The aim of the present study was to investigate whether titanium (Ti)-induced release of interleukin (IL)-1ß acts through the assembly of the NACHT, LRR, and PYD domain-containing protein 3 (NLRP3) inflammasome. In addition, we examined whether particulate Ti or TiO2 activates the same intracellular pathways with the assembly of the NLRP3 inflammasome as Ti ions. Ti ions are known to induce IL-1ß maturation and release by the formation of metal-protein aggregates. Wild-type THP-1 (wt.) cells and NLRP3- and ASC- (apoptosis-associated speck-like protein containing caspase recruitment domain (CARD)) knockdown cells were used in the experimental analyses. Macro- and nanoparticles (NPs) of both Ti and TiO2 were used as test agents. IL-1ß release as a biomarker for inflammasome activation and cell viability was also analyzed. Periodate-oxidized adenosine triphosphate (oATP) was used to attenuate downstream signaling in NLRP3 inflammasome activation. Cellular uptake of Ti was examined using transmission electron microscopy. Cells exposed to the Ti-ion solution showed a dose-dependent increase in the release of IL-1ß; conversely, exposure to particulate Ti did not result in increased IL-1ß release. Cell viability was not affected by particulate Ti. Knockdown cells exposed to Ti showed a statistically significant reduction in the release of IL-1ß compared with wt. cells (p < 0.001). Cellular uptake was detected in all Ti mixtures, and aggregates with various structures were observed. Ti ion-induced release of bioactive IL-1ß in THP-1 cells involves the assembly of the NLRP3 inflammasome.

Asunto(s)

Inflamasomas; Interleucina-1beta; Macrófagos; Proteína con Dominio Pirina 3 de la Familia NLR; Titanio; Biomarcadores/metabolismo; Caspasa 1/metabolismo; Humanos; Inflamasomas/efectos de los fármacos; Inflamasomas/metabolismo; Interleucina-1beta/genética; Interleucina-1beta/metabolismo; Iones/metabolismo; Macrófagos/efectos de los fármacos; Macrófagos/metabolismo; Proteína con Dominio Pirina 3 de la Familia NLR/genética; Proteína con Dominio Pirina 3 de la Familia NLR/metabolismo; Transportadores de Anión Orgánico/metabolismo; Agregado de Proteínas; Células THP-1; Titanio/farmacología

Palabras clave

Interleukin-1ß; Macrophage cell line; NLRP3 inflammasome; Titanium

Texto completo

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Titanio / Interleucina-1beta / Inflamasomas / Proteína con Dominio Pirina 3 de la Familia NLR / Macrófagos Límite: Humans Idioma: En Revista: Inflammation Año: 2022 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Estados Unidos

Texto completo

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Buscar en Google