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Magnesium(II)-ATP Complexes in 1-Ethyl-3-Methylimidazolium Acetate Solutions Characterized by 31 Mg ß-Radiation-Detected NMR Spectroscopy.
McFadden, Ryan M L; Szunyogh, Dániel; Bravo-Frank, Nicholas; Chatzichristos, Aris; Dehn, Martin H; Fujimoto, Derek; Jancsó, Attila; Johannsen, Silke; Kálomista, Ildikó; Karner, Victoria L; Kiefl, Robert F; Larsen, Flemming H; Lassen, Jens; Levy, C D Philip; Li, Ruohong; McKenzie, Iain; McPhee, Hannah; Morris, Gerald D; Pearson, Matthew R; Sauer, Stephan P A; Sigel, Roland K O; Thulstrup, Peter W; MacFarlane, W Andrew; Hemmingsen, Lars; Stachura, Monika.
Afiliación
  • McFadden RML; TRIUMF, 4004 Wesbrook Mall, Vancouver, BC V6T 2A3, Canada.
  • Szunyogh D; Department of Chemistry, University of Copenhagen, Universitetsparken 5, 2100, Copenhagen, Denmark.
  • Bravo-Frank N; Faculty of Engineering, University of Victoria, 3800 Finnerty Road, Victoria, BC V8P 5C2, Canada.
  • Chatzichristos A; Department of Physics and Astronomy, University of British Columbia, 6224 Agricultural Road, Vancouver, BC V6T 1Z1, Canada.
  • Dehn MH; Stewart Blusson Quantum Matter Institute, University of British Columbia, 2355 East Mall, Vancouver, BC V6T 1Z4, Canada.
  • Fujimoto D; Department of Physics and Astronomy, University of British Columbia, 6224 Agricultural Road, Vancouver, BC V6T 1Z1, Canada.
  • Jancsó A; Stewart Blusson Quantum Matter Institute, University of British Columbia, 2355 East Mall, Vancouver, BC V6T 1Z4, Canada.
  • Johannsen S; Department of Physics and Astronomy, University of British Columbia, 6224 Agricultural Road, Vancouver, BC V6T 1Z1, Canada.
  • Kálomista I; Stewart Blusson Quantum Matter Institute, University of British Columbia, 2355 East Mall, Vancouver, BC V6T 1Z4, Canada.
  • Karner VL; Department of Inorganic and Analytical Chemistry, University of Szeged, Dóm tér 7, 6720, Szeged, Hungary.
  • Kiefl RF; Department of Chemistry, University of Zurich, Winterthurerstrasse 190, 8057, Zürich, Switzerland.
  • Larsen FH; Department of Chemistry, University of Copenhagen, Universitetsparken 5, 2100, Copenhagen, Denmark.
  • Lassen J; Stewart Blusson Quantum Matter Institute, University of British Columbia, 2355 East Mall, Vancouver, BC V6T 1Z4, Canada.
  • Levy CDP; Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, BC V6T 1Z1, Canada.
  • Li R; TRIUMF, 4004 Wesbrook Mall, Vancouver, BC V6T 2A3, Canada.
  • McKenzie I; Department of Physics and Astronomy, University of British Columbia, 6224 Agricultural Road, Vancouver, BC V6T 1Z1, Canada.
  • McPhee H; Stewart Blusson Quantum Matter Institute, University of British Columbia, 2355 East Mall, Vancouver, BC V6T 1Z4, Canada.
  • Morris GD; Department of Food Science, University of Copenhagen, Rolighedsvej 26, 1958, Frederiksberg, Denmark.
  • Pearson MR; TRIUMF, 4004 Wesbrook Mall, Vancouver, BC V6T 2A3, Canada.
  • Sauer SPA; Department of Physics, Simon Fraser University, 8888 University Drive, Burnaby, BC V5A 1S6, Canada.
  • Sigel RKO; Department of Physics and Astronomy, University of Manitoba, 30A Sifton Road, Winnipeg, MB R3T 2N2, Canada.
  • Thulstrup PW; TRIUMF, 4004 Wesbrook Mall, Vancouver, BC V6T 2A3, Canada.
  • MacFarlane WA; TRIUMF, 4004 Wesbrook Mall, Vancouver, BC V6T 2A3, Canada.
  • Hemmingsen L; TRIUMF, 4004 Wesbrook Mall, Vancouver, BC V6T 2A3, Canada.
  • Stachura M; Department of Chemistry, Simon Fraser University, 8888 University Drive, Burnaby, BC V5A 1S6, Canada.
Angew Chem Int Ed Engl ; 61(35): e202207137, 2022 08 26.
Article en En | MEDLINE | ID: mdl-35718746
The complexation of MgII with adenosine 5'-triphosphate (ATP) is omnipresent in biochemical energy conversion, but is difficult to interrogate directly. Here we use the spin- 1/2 ß-emitter 31 Mg to study MgII -ATP complexation in 1-ethyl-3-methylimidazolium acetate (EMIM-Ac) solutions using ß-radiation-detected nuclear magnetic resonance (ß-NMR). We demonstrate that (nuclear) spin-polarized 31 Mg, following ion-implantation from an accelerator beamline into EMIM-Ac, binds to ATP within its radioactive lifetime before depolarizing. The evolution of the spectra with solute concentration indicates that the implanted 31 Mg initially bind to the solvent acetate anions, whereafter they undergo dynamic exchange and form either a mono- (31 Mg-ATP) or di-nuclear (31 MgMg-ATP) complex. The chemical shift of 31 Mg-ATP is observed up-field of 31 MgMg-ATP, in accord with quantum chemical calculations. These observations constitute a crucial advance towards using ß-NMR to probe chemistry and biochemistry in solution.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Adenosina Trifosfato / Magnesio Idioma: En Revista: Angew Chem Int Ed Engl Año: 2022 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Alemania
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Adenosina Trifosfato / Magnesio Idioma: En Revista: Angew Chem Int Ed Engl Año: 2022 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Alemania