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Topolin cytokinins enhanced shoot proliferation, reduced hyperhydricity and altered cytokinin metabolism in Pistacia vera L. seedling explants.
Abdouli, Dhekra; Placková, Lenka; Dolezal, Karel; Bettaieb, Taoufik; Werbrouck, Stefaan P O.
Afiliación
  • Abdouli D; Laboratory for Applied In Vitro Plant Biotechnology, University Ghent, Valentin Vaerwyckweg 1, 9000 Ghent, Belgium; Laboratory of Horticultural Sciences, University of Carthage, National Agronomic Institute of Tunisia, 43 Av. Charles Nicolle, 1082 Tunis, Tunisia.
  • Placková L; Laboratory of Growth Regulators, Palacky University and Institute of Experimental Botany, AS CR, Slechtitelu 11, 783 71 Olomouc, Czech Republic.
  • Dolezal K; Laboratory of Growth Regulators, Palacky University and Institute of Experimental Botany, AS CR, Slechtitelu 11, 783 71 Olomouc, Czech Republic; Department of Chemical Biology, Faculty of Science, Palacký University, Slechtitelu 27, CZ-78371 Olomouc, Czech Republic.
  • Bettaieb T; Laboratory of Horticultural Sciences, University of Carthage, National Agronomic Institute of Tunisia, 43 Av. Charles Nicolle, 1082 Tunis, Tunisia.
  • Werbrouck SPO; Laboratory for Applied In Vitro Plant Biotechnology, University Ghent, Valentin Vaerwyckweg 1, 9000 Ghent, Belgium. Electronic address: stefaan.werbrouck@ugent.be.
Plant Sci ; 322: 111360, 2022 Sep.
Article en En | MEDLINE | ID: mdl-35716901
The effect of 10 µM meta-topolin (mT) and meta-topolin riboside (mTR) on in vitro proliferation and anomalies of Pistacia vera L. were evaluated and compared to that of 6-benzylaminopurine (BA). The highest proliferation rate (15.6) was recorded in the mT-medium, with a value 6 times higher than in BA-medium. Moreover, the lowest percentage of hyperhydric usable shoots (58,9%) and callus weight (46,9%) were found in mTR-treated shoots. Shoot tip as well as leaf necrosis were not influenced by cytokinin (CK) type. Image analysis was used to evaluate photosynthetic efficiency as well as anthocyanin index. Photosynthesis was more efficient with BA and mTR but the higher anthocyanin accumulation in BA-treated shoots suggests more stress. Endogenous CKs and their metabolites were determined in seedlings and, for the first time, the metabolism of exogenous BA, mT and mTR was studied in pistachio. The stimulating effect on cis-zeatin and its riboside and the appearance of BA and traces of ortho-topolin and para-topolin as natural CKs are discussed. The quantitative and qualitative CK metabolite analyses provides some initial clues as to why topolin would be superior to BA in terms of proliferation rate and avoiding hyperhydricity and allowed a better understanding of the effect of exogenous administration of CK.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pistacia / Citocininas Tipo de estudio: Qualitative_research Idioma: En Revista: Plant Sci Año: 2022 Tipo del documento: Article País de afiliación: Túnez Pais de publicación: Irlanda
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pistacia / Citocininas Tipo de estudio: Qualitative_research Idioma: En Revista: Plant Sci Año: 2022 Tipo del documento: Article País de afiliación: Túnez Pais de publicación: Irlanda