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Imaging peripheral nerve micro-anatomy with MUSE, 2D and 3D approaches.
Kolluru, Chaitanya; Todd, Austin; Upadhye, Aniruddha R; Liu, Yehe; Berezin, Mikhail Y; Fereidouni, Farzad; Levenson, Richard M; Wang, Yanming; Shoffstall, Andrew J; Jenkins, Michael W; Wilson, David L.
Afiliación
  • Kolluru C; Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH, 44106, USA.
  • Todd A; University of Texas Health Science Center at San Antonio, San Antonio, TX, 78229, USA.
  • Upadhye AR; Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH, 44106, USA.
  • Liu Y; APT Center, Louis Stokes Cleveland VA Medical Center, Cleveland, OH, 44106, USA.
  • Berezin MY; Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH, 44106, USA.
  • Fereidouni F; Department of Radiology, Washington University in St. Louis, St. Louis, MO, 63110, USA.
  • Levenson RM; Department of Pathology and Laboratory Medicine, UC Davis Health, Sacramento, CA, 95817, USA.
  • Wang Y; Department of Pathology and Laboratory Medicine, UC Davis Health, Sacramento, CA, 95817, USA.
  • Shoffstall AJ; Department of Radiology, Case Western Reserve University, Cleveland, OH, 44106, USA.
  • Jenkins MW; Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH, 44106, USA.
  • Wilson DL; APT Center, Louis Stokes Cleveland VA Medical Center, Cleveland, OH, 44106, USA.
Sci Rep ; 12(1): 10205, 2022 06 17.
Article en En | MEDLINE | ID: mdl-35715554
Understanding peripheral nerve micro-anatomy can assist in the development of safe and effective neuromodulation devices. However, current approaches for imaging nerve morphology at the fiber level are either cumbersome, require substantial instrumentation, have a limited volume of view, or are limited in resolution/contrast. We present alternative methods based on MUSE (Microscopy with Ultraviolet Surface Excitation) imaging to investigate peripheral nerve morphology, both in 2D and 3D. For 2D imaging, fixed samples are imaged on a conventional MUSE system either label free (via auto-fluorescence) or after staining with fluorescent dyes. This method provides a simple and rapid technique to visualize myelinated nerve fibers at specific locations along the length of the nerve and perform measurements of fiber morphology (e.g., axon diameter and g-ratio). For 3D imaging, a whole-mount staining and MUSE block-face imaging method is developed that can be used to characterize peripheral nerve micro-anatomy and improve the accuracy of computational models in neuromodulation. Images of rat sciatic and human cadaver tibial nerves are presented, illustrating the applicability of the method in different preclinical models.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nervios Periféricos / Alprostadil Límite: Animals Idioma: En Revista: Sci Rep Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nervios Periféricos / Alprostadil Límite: Animals Idioma: En Revista: Sci Rep Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido