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Antimicrobial effects of agitational irrigation on single- and multispecies biofilms in dentin canals.
Al-Zuhair, Hind; Su, Zheng; Liu, He; Wang, Zhejun; Haapasalo, Markus; Hieawy, Ahmed; Gao, Yuan; Shen, Ya.
Afiliación
  • Al-Zuhair H; Division of Endodontics, Department of Oral Biological and Medical Sciences, Faculty of Dentistry, University of British Columbia, 2199 Wesbrook Mall, Vancouver, BC, V6T 1Z3, Canada.
  • Su Z; Division of Endodontics, Department of Oral Biological and Medical Sciences, Faculty of Dentistry, University of British Columbia, 2199 Wesbrook Mall, Vancouver, BC, V6T 1Z3, Canada.
  • Liu H; Department of Endodontics, Beijing Stomatological Hospital, Capital Medical University, Beijing, China.
  • Wang Z; Division of Endodontics, Department of Oral Biological and Medical Sciences, Faculty of Dentistry, University of British Columbia, 2199 Wesbrook Mall, Vancouver, BC, V6T 1Z3, Canada.
  • Haapasalo M; Department of Stomatology, Affiliated Hospital of Jining Medical University, Jining, China.
  • Hieawy A; Division of Endodontics, Department of Oral Biological and Medical Sciences, Faculty of Dentistry, University of British Columbia, 2199 Wesbrook Mall, Vancouver, BC, V6T 1Z3, Canada.
  • Gao Y; Division of Endodontics, Department of Oral Biological and Medical Sciences, Faculty of Dentistry, University of British Columbia, 2199 Wesbrook Mall, Vancouver, BC, V6T 1Z3, Canada.
  • Shen Y; Division of Endodontics, Department of Oral Biological and Medical Sciences, Faculty of Dentistry, University of British Columbia, 2199 Wesbrook Mall, Vancouver, BC, V6T 1Z3, Canada.
Odontology ; 111(1): 49-56, 2023 Jan.
Article en En | MEDLINE | ID: mdl-35713740
This study aimed to compare the antibacterial effects of different agitation devices on single- and multispecies biofilms in dentin canals using confocal laser scanning microscopy (CLSM). Dentin blocks were prepared from human root dentin. Enterococcus faecalis and multiple species were introduced into the dentinal tubules via centrifugation and incubation. Two infected dentin samples were placed at 8 and 16 mm in a customized model. Samples were randomly divided into eight groups according to the agitation device used: syringe needle irrigation, EndoActivator, passive ultrasonic irrigation (PUI), and EDDY, at 2.5% or 6% NaOCl concentrations. The samples were stained and observed using CLSM. Statistical analysis was performed using an independent sample t test and analysis of variance. Linear models were used to assess the joint impact of the experimental groups on the proportion of biofilms killed. No significant differences were observed between the killing rates of the single- and multispecies biofilms. Both concentrations of NaOCl significantly increased the percentage of dead bacteria compared with the control. Biofilms in dentin tubules was more effectively killed when NaOCl was agitated; however, the difference between PUI and EDDY was not significant. Significantly more bacteria were killed in dentin blocks placed at 8 mm than at 16 mm (p < 0.05). In conclusion, EDDY was as effective as PUI when combined with NaOCl. However, the apical portion, which had a low antimicrobial efficiency, remains a concern. Mechanical instrumentation is incapable of completely eradicating bacteria, and additional research is required to improve the efficacy of root canal disinfection.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cavidad Pulpar / Dentina Límite: Humans Idioma: En Revista: Odontology Asunto de la revista: ODONTOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Japón
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cavidad Pulpar / Dentina Límite: Humans Idioma: En Revista: Odontology Asunto de la revista: ODONTOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Japón