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Influence of very-long-chain polyunsaturated fatty acids on membrane structure and dynamics.
Cheng, Victoria; Rallabandi, Rameshu; Gorusupudi, Aruna; Lucas, Steven; Rognon, Gregory; Bernstein, Paul S; Rainier, Jon D; Conboy, John C.
Afiliación
  • Cheng V; Department of Chemistry, University of Utah, Salt Lake City, Utah.
  • Rallabandi R; Department of Chemistry, University of Utah, Salt Lake City, Utah.
  • Gorusupudi A; Department of Ophthalmology and Visual Sciences, John A. Moran Eye Center, University of Utah, Salt Lake City, Utah.
  • Lucas S; Department of Chemistry, University of Utah, Salt Lake City, Utah.
  • Rognon G; Department of Ophthalmology and Visual Sciences, John A. Moran Eye Center, University of Utah, Salt Lake City, Utah.
  • Bernstein PS; Department of Ophthalmology and Visual Sciences, John A. Moran Eye Center, University of Utah, Salt Lake City, Utah.
  • Rainier JD; Department of Chemistry, University of Utah, Salt Lake City, Utah.
  • Conboy JC; Department of Chemistry, University of Utah, Salt Lake City, Utah. Electronic address: conboy@chem.utah.edu.
Biophys J ; 121(14): 2730-2741, 2022 07 19.
Article en En | MEDLINE | ID: mdl-35711144
The unique attributes of very-long-chain polyunsaturated fatty acids (VLC-PUFAs), their long carbon chains (n > 24) and high degree of unsaturation, impart unique chemical and physical properties to this class of fatty acids. The changes imparted by VLC-PUFA 32:6 n-3 on lipid packing and the compression moduli of model membranes were evaluated from π-A isotherms of VLC-PUFA in 1,2-distearoyl-sn-3-glycero-phosphocholine (DSPC) lipid monolayers. To compare the attractive or repulsive forces between VLC-PUFA and DSPC lipid monolayers, the measured mean molecular areas (MMAs) were compared with the calculated MMAs of an ideal mixture of VLC-PUFA and DSPC. The presence of 0.1, 1, and 10 mol % VLC-PUFA shifted the π-A isotherm to higher MMAs of the lipids comprising the membrane and the observed positive deviations from ideal behavior of the mixed VLC-PUFA:DSPC monolayers correspond to repulsive forces between VLC-PUFAs and DSPC. The MMA of the VLC-PUFA component was estimated using the measured MMAs of DSPC of 47.1 ± 0.7 Å2/molecule, to be 15,000, 1100, and 91 Å2/molecule at 0.1, 1, and 10 mol % VLC-PUFA:DSPC mixtures, respectively. The large MMAs of VLC-PUFA suggest that the docosahexaenoic acid tail reinserts into the membrane and adopts a nonlinear structure in the membrane, which is most pronounced at 0.1 mol % VLC-PUFA. The presence of 0.1 mol % VLC-PUFA:DSPC also significantly increased the compression modulus of the membrane by 28 mN/m compared with a pure DSPC membrane. The influence of VLC-PUFA on lipid "flip-flop" was investigated by sum-frequency vibrational spectroscopy. The incorporation of 0.1 mol % VLC-PUFA increased the DSPC flip-flop rate fourfold. The fact that VLC-PUFA promotes lipid translocation is noteworthy as retinal membranes require a high influx of retinoids which may be facilitated by lipid flip-flop.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosfatidilcolinas / Ácidos Grasos Idioma: En Revista: Biophys J Año: 2022 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosfatidilcolinas / Ácidos Grasos Idioma: En Revista: Biophys J Año: 2022 Tipo del documento: Article Pais de publicación: Estados Unidos