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Mass spectrometry-based draft of the mouse proteome.
Giansanti, Piero; Samaras, Patroklos; Bian, Yangyang; Meng, Chen; Coluccio, Andrea; Frejno, Martin; Jakubowsky, Hannah; Dobiasch, Sophie; Hazarika, Rashmi R; Rechenberger, Julia; Calzada-Wack, Julia; Krumm, Johannes; Mueller, Sebastian; Lee, Chien-Yun; Wimberger, Nicole; Lautenbacher, Ludwig; Hassan, Zonera; Chang, Yun-Chien; Falcomatà, Chiara; Bayer, Florian P; Bärthel, Stefanie; Schmidt, Tobias; Rad, Roland; Combs, Stephanie E; The, Matthew; Johannes, Frank; Saur, Dieter; de Angelis, Martin Hrabe; Wilhelm, Mathias; Schneider, Günter; Kuster, Bernhard.
Afiliación
  • Giansanti P; Chair of Proteomics and Bioanalytics, Technical University of Munich, Freising, Germany.
  • Samaras P; Chair of Proteomics and Bioanalytics, Technical University of Munich, Freising, Germany.
  • Bian Y; Chair of Proteomics and Bioanalytics, Technical University of Munich, Freising, Germany.
  • Meng C; College of Life Science, Northwest University, Xi'an, China.
  • Coluccio A; Bavarian Biomolecular Mass Spectrometry Center, Technical University of Munich, Freising, Germany.
  • Frejno M; Division of Translational Cancer Research, German Cancer Research Center (DKFZ) and German Cancer Consortium (DKTK), Heidelberg, Germany.
  • Jakubowsky H; Chair of Translational Cancer Research and Institute for Experimental Cancer Therapy, Klinikum rechts der Isar, School of Medicine, Technical University of Munich, Munich, Germany.
  • Dobiasch S; Department of Internal Medicine II, Klinikum rechts der Isar, Technical University of Munich, Munich, Germany.
  • Hazarika RR; Center for Translational Cancer Research (TranslaTUM), School of Medicine, Technical University of Munich, Munich, Germany.
  • Rechenberger J; Chair of Proteomics and Bioanalytics, Technical University of Munich, Freising, Germany.
  • Calzada-Wack J; Division of Translational Cancer Research, German Cancer Research Center (DKFZ) and German Cancer Consortium (DKTK), Heidelberg, Germany.
  • Krumm J; Chair of Translational Cancer Research and Institute for Experimental Cancer Therapy, Klinikum rechts der Isar, School of Medicine, Technical University of Munich, Munich, Germany.
  • Mueller S; Department of Internal Medicine II, Klinikum rechts der Isar, Technical University of Munich, Munich, Germany.
  • Lee CY; Center for Translational Cancer Research (TranslaTUM), School of Medicine, Technical University of Munich, Munich, Germany.
  • Wimberger N; Department of Radiation Oncology, Klinikum rechts der Isar, Technical University of Munich, Munich, Germany.
  • Lautenbacher L; Institute of Radiation Medicine, Department of Radiation Sciences, Helmholtz Zentrum München, Neuherberg, Germany.
  • Hassan Z; German Cancer Consortium (DKTK), Munich, Germany.
  • Chang YC; German Cancer Research Center (DKFZ), Heidelberg, Germany.
  • Falcomatà C; Population epigenetics and epigenomics, Technical University of Munich, Freising, Germany.
  • Bayer FP; Institute of Advanced Study (IAS), Technical University of Munich, Freising, Germany.
  • Bärthel S; Chair of Proteomics and Bioanalytics, Technical University of Munich, Freising, Germany.
  • Schmidt T; Institute of Experimental Genetics, German Mouse Clinic, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany.
  • Rad R; Chair of Proteomics and Bioanalytics, Technical University of Munich, Freising, Germany.
  • Combs SE; Department of Internal Medicine II, Klinikum rechts der Isar, Technical University of Munich, Munich, Germany.
  • The M; Center for Translational Cancer Research (TranslaTUM), School of Medicine, Technical University of Munich, Munich, Germany.
  • Johannes F; Institute of Molecular Oncology and Functional Genomics, School of Medicine, Technical University of Munich, Munich, Germany.
  • Saur D; Chair of Proteomics and Bioanalytics, Technical University of Munich, Freising, Germany.
  • de Angelis MH; Division of Translational Cancer Research, German Cancer Research Center (DKFZ) and German Cancer Consortium (DKTK), Heidelberg, Germany.
  • Wilhelm M; Chair of Translational Cancer Research and Institute for Experimental Cancer Therapy, Klinikum rechts der Isar, School of Medicine, Technical University of Munich, Munich, Germany.
  • Schneider G; Department of Internal Medicine II, Klinikum rechts der Isar, Technical University of Munich, Munich, Germany.
  • Kuster B; Center for Translational Cancer Research (TranslaTUM), School of Medicine, Technical University of Munich, Munich, Germany.
Nat Methods ; 19(7): 803-811, 2022 07.
Article en En | MEDLINE | ID: mdl-35710609
The laboratory mouse ranks among the most important experimental systems for biomedical research and molecular reference maps of such models are essential informational tools. Here, we present a quantitative draft of the mouse proteome and phosphoproteome constructed from 41 healthy tissues and several lines of analyses exemplify which insights can be gleaned from the data. For instance, tissue- and cell-type resolved profiles provide protein evidence for the expression of 17,000 genes, thousands of isoforms and 50,000 phosphorylation sites in vivo. Proteogenomic comparison of mouse, human and Arabidopsis reveal common and distinct mechanisms of gene expression regulation and, despite many similarities, numerous differentially abundant orthologs that likely serve species-specific functions. We leverage the mouse proteome by integrating phenotypic drug (n > 400) and radiation response data with the proteomes of 66 pancreatic ductal adenocarcinoma (PDAC) cell lines to reveal molecular markers for sensitivity and resistance. This unique atlas complements other molecular resources for the mouse and can be explored online via ProteomicsDB and PACiFIC.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Pancreáticas / Arabidopsis / Carcinoma Ductal Pancreático Límite: Animals Idioma: En Revista: Nat Methods Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2022 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Pancreáticas / Arabidopsis / Carcinoma Ductal Pancreático Límite: Animals Idioma: En Revista: Nat Methods Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2022 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos