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Immunotherapeutic effects of recombinant colorectal cancer antigen produced in tomato fruits.
Park, Se Hee; Ji, Kon-Young; Park, Seo Young; Kim, Hyun Min; Ma, Sang Hoon; Do, Ju Hui; Kang, Hyuno; Kang, Hyung Sik; Oh, Doo-Byoung; Shim, Jae Sung; Joung, Young Hee.
Afiliación
  • Park SH; School of Biological Sciences and Technology, Chonnam National University, Gwangju, 61186, Korea.
  • Ji KY; Herbal Medicine Research Division, Korea Institute of Oriental Medicine, Daejeon, 34054, Korea.
  • Park SY; School of Biological Sciences and Technology, Chonnam National University, Gwangju, 61186, Korea.
  • Kim HM; School of Biological Sciences and Technology, Chonnam National University, Gwangju, 61186, Korea.
  • Ma SH; School of Biological Sciences and Technology, Chonnam National University, Gwangju, 61186, Korea.
  • Do JH; School of Biological Sciences and Technology, Chonnam National University, Gwangju, 61186, Korea.
  • Kang H; Division of Analytical Science, Korea Basic Science Institute (KBSI), Daejeon, 34133, Republic of Korea.
  • Kang HS; School of Biological Sciences and Technology, Chonnam National University, Gwangju, 61186, Korea.
  • Oh DB; Environmental Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141, Korea.
  • Shim JS; Department of Biosystems and Bioengineering, KRIBB School of Biotechnology, University of Science and Technology (UST), Daejeon, 34113, Korea.
  • Joung YH; School of Biological Sciences and Technology, Chonnam National University, Gwangju, 61186, Korea. jsshim@chonnam.ac.kr.
Sci Rep ; 12(1): 9723, 2022 06 13.
Article en En | MEDLINE | ID: mdl-35697846
The production of pharmacological vaccines in plants has been an important goal in the field of plant biotechnology. GA733-2, the protein that is also known as colorectal carcinoma (CRC)-associated antigen, is a strong candidate to produce a colorectal cancer vaccine. Tomato is the one of the major targets for production of an edible vaccine, as tomato is a fruit consumed in fresh form. It also contains high content of vitamins that aid activation of immune response. In order to develop an edible colorectal cancer vaccine, the transgene rGA733-Fc that encodes a fusion protein of GA733-2, the fragment crystallizable (Fc) domain, and the ER retention motif (rGA733-Fc) was introduced into tomato plants (Solanum lycopersicum cv. Micro-Tom). The transgenic plants producing rGA733-Fc (rGA733-FcOX) protein were screened based on stable integration of transgene expression cassette and expression level of rGA733-Fc protein. Further glycosylation pattern analysis revealed that plant derived rGA733-Fc protein contains an oligomannose glycan structure, which is a typical glycosylation pattern found on ER-processing proteins. The red fruits of rGA733-FcOX transgenic tomato plants containing approximately 270 ng/g FW of rGA733-Fc protein were orally administered to C57BL/6 mice. Oral administration of tomato fruits of the rGA733-Fc expressing transgenic plants delayed colorectal cancer growth and stimulated immune responses compared to oral administration of tomato fruits of the h-Fc expressing transgenic plants in the C57BL/6J mice. This is the first study showing the possibility of producing an edible colorectal cancer vaccine using tomato plants. This research would be helpful for development of plant-derived cancer edible vaccines.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Colorrectales / Solanum lycopersicum Límite: Animals Idioma: En Revista: Sci Rep Año: 2022 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Colorrectales / Solanum lycopersicum Límite: Animals Idioma: En Revista: Sci Rep Año: 2022 Tipo del documento: Article Pais de publicación: Reino Unido