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Co-administration of chloroquine and coenzyme Q10 improved treatment outcome during experimental cerebral malaria.
Ouko, David B; Amwayi, Peris W; Ochola, Lucy A; Wairagu, Peninah M; Isaac, Alfred Orina; Nyariki, James N.
Afiliación
  • Ouko DB; Department of Biochemistry and Biotechnology, The Technical of University of Kenya, P.O Box, Nairobi, 52428-00200 Kenya.
  • Amwayi PW; Department of Biochemistry and Biotechnology, The Technical of University of Kenya, P.O Box, Nairobi, 52428-00200 Kenya.
  • Ochola LA; Department of Tropical and Infectious Diseases, Institute of Primate Research, P.O Box, Karen, 24481-00502 Kenya.
  • Wairagu PM; Department of Biochemistry and Biotechnology, The Technical of University of Kenya, P.O Box, Nairobi, 52428-00200 Kenya.
  • Isaac AO; Department of Pharmaceutical Sciences and Technology - School Health Sciences and Biomedical Sciences, The Technical University of Kenya, P.O Box, Nairobi, 52428-00200 Kenya.
  • Nyariki JN; Department of Biochemistry and Biotechnology, The Technical of University of Kenya, P.O Box, Nairobi, 52428-00200 Kenya.
J Parasit Dis ; 46(2): 466-475, 2022 Jun.
Article en En | MEDLINE | ID: mdl-35692470
Development of cerebral malaria (CM) is driven by parasitemia levels, harmful inflammatory response, oxidative stress and consequent breach of the blood brain barrier. Use of adjunct therapy that utilizes an antioxidant and anti-inflammatory agent alongside chloroquine (CQ), may improve treatment outcome and shorten recovery from post-infection sequelae. Though withdrawn in some countries, CQ is still in use for prophylaxis and treatment of malaria in many countries. Current study investigated whether oral co-administration of 50 mg/kg CQ and 200 mg/kg of coenzyme Q10 (CoQ10) would improve treatment outcome against experimental cerebral malaria (ECM) and assuage the deleterious effects of oxidative stress and inflammation upon infection by Plasmodium berghei ANKA (PbA) in a C57BL/6 J mouse model. Treatment with CQ + CoQ10 resulted in an improved parasite elimination; clearing the parasite one day early, when compared to mice on CQ alone. Remarkably, treatment with CQ and CoQ10 separately or in combination, assuaged PbA induced elevation of serum levels of TNF-α and IFN-γ an indication of protection from ECM progression. Furthermore, CQ and CoQ10-administration, blocked parasite-driven elevation of aspartate transaminase (AST), alanine transaminase (ALT) and bilirubin. In the presence of CQ and CoQ10, severe PbA-induced systemic induction of oxidative stress and resultant GSH depletion was reduced in the brain, liver, spleen, and kidney. Overall, these findings demonstrate that administration of CQ and CoQ10 ameliorates harmful parasite-driven oxidative stress and inflammation, while slowing the progression to full blown ECM and may improve treatment outcome in CM.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: J Parasit Dis Año: 2022 Tipo del documento: Article Pais de publicación: India

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: J Parasit Dis Año: 2022 Tipo del documento: Article Pais de publicación: India