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Development of a microfluidic droplet platform with an antibody-free magnetic-bead-based strategy for high through-put and efficient EVs isolation.
Morani, Marco; Taverna, Myriam; Krupova, Zuzana; Alexandre, Lucile; Defrenaix, Pierre; Mai, Thanh Duc.
Afiliación
  • Morani M; Université Paris-Saclay, CNRS, Institut Galien Paris-Saclay, 92296, Châtenay-Malabry, France.
  • Taverna M; Université Paris-Saclay, CNRS, Institut Galien Paris-Saclay, 92296, Châtenay-Malabry, France; Institut Universitaire de France (IUF), France.
  • Krupova Z; Excilone - 6, Rue Blaise Pascal - Parc Euclide, 78990, Elancourt, France.
  • Alexandre L; Université Paris-Saclay, CNRS, Institut Galien Paris-Saclay, 92296, Châtenay-Malabry, France; Laboratoire Physico Chimie Curie, Institut Curie, PSL Research University, CNRS UMR168, Paris, France.
  • Defrenaix P; Excilone - 6, Rue Blaise Pascal - Parc Euclide, 78990, Elancourt, France.
  • Mai TD; Université Paris-Saclay, CNRS, Institut Galien Paris-Saclay, 92296, Châtenay-Malabry, France. Electronic address: thanh-duc.mai@universite-paris-saclay.fr.
Talanta ; 249: 123625, 2022 Nov 01.
Article en En | MEDLINE | ID: mdl-35688075
In this study, we present a novel microfluidic droplet-based strategy for high performance isolation of extracellular vesicles (EVs). For EVs capture and release, a magnetic bead-based approach without having recourse to any antibody was optimized in batch and then adapted to the microfluidic droplet system. This antibody-free capture approach relies on the presence of a water-excluding polymer, polyethylene glycol (PEG), to precipitate EVs on the surface of negatively charged magnetic beads. We significantly improved the reproducibility of EV recovery and avoided positive false bias by including a washing step and optimizing the protocol. Well-characterized EV standards derived from pre-purified bovine milk were used for EVs isolation performance evaluation. An EVs recovery of up to 25% estimated with nanoparticle tracking analysis (NTA) was achieved for this batchwise PEG-based approach. The confirmation of isolated EVs identity was also made with our recently developed method using capillary electrophoresis (CE) coupled with laser-induced fluorescent (LIF) detection. In parallel, a purpose-made droplet platform working with magnetic tweezers was developed for translation of this PEG-based method into a droplet microfluidic protocol to further improve the performance in terms of EVs capture efficiency and high throughput. The droplet-based protocol offers a significant improvement of recovery rate (up to 50%) while reducing sample and reagent volumes (by more than 10 folds) and operation time (by 3 folds) compared to the batch-wise mode.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Microfluídica / Vesículas Extracelulares Idioma: En Revista: Talanta Año: 2022 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Microfluídica / Vesículas Extracelulares Idioma: En Revista: Talanta Año: 2022 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Países Bajos