Your browser doesn't support javascript.
loading
Effect of lentivirus-mediated peroxiredoxins 6 gene silencing on the phenotype of human gastric cancer BGC-823 cells.
Mu, RunHong; Li, YuPeng; Xing, JiaYing; Li, YanDong; Lin, Rui; Ye, SiPing; Zhang, YaoYue; Mu, Han; Guo, Xiao; An, LiPing.
Afiliación
  • Mu R; Basic Medical College of Beihua University, Affiliated Hospital of Beihua University, Jilin, Jilin, P. R. China.
  • Li Y; Basic Medical College of Beihua University, Affiliated Hospital of Beihua University, Jilin, Jilin, P. R. China.
  • Xing J; Pharmacy College of Beihua University, Jilin, Jilin, P. R. China.
  • Li Y; Department of General Surgery, Affiliated Hospital of Beihua University, Jilin, Jilin, P. R. China.
  • Lin R; Pharmacy College of Beihua University, Jilin, Jilin, P. R. China.
  • Ye S; Pharmacy College of Beihua University, Jilin, Jilin, P. R. China.
  • Zhang Y; Stomatology College of Beihua University, Jilin, Jilin, P. R. China.
  • Mu H; Laboratory Medical College of Beihua University, Jilin, Jilin, P. R. China.
  • Guo X; Pharmacy College of Beihua University, Jilin, Jilin, P. R. China.
  • An L; Pharmacy College of Beihua University, Jilin, Jilin, P. R. China.
J Cancer Res Ther ; 18(2): 411-417, 2022 Apr.
Article en En | MEDLINE | ID: mdl-35645108
Aims: Peroxiredoxins (PRDX6) regulates the occurrence and progression of cancer. The aim of this study is to investigate the effect of PRDX6 knockdown on the biological behavior of human gastric cancer cell line BGC-823 cells. Settings and Design: Research article. Subjects and Methods: The differential expression of PRDX6 in gastric cancer and normal gastric tissues was tested by immunohistochemistry. Ribonucleic acid plasmid of PRDX6 gene was packaged using a lentivirus, and BGC-823 cells were transfected with the lentivirus to obtain a BGC-823 cell line in which the expression of PRDX6 was stably silenced. Statistical Analysis Used: The proliferation activity of BGC-823 cells was detected using the cell counting kit-8 method. The effect of PRDX6 on the migration and invasion of BGC-823 cells was evaluated using the scratch test and Transwell assay, and the expression of related proteins was detected by western blot. Results: The expression of PRDX6 in gastric cancer was significantly increased (P < 0.05). Compared with those in the untransfected and negative control groups. The proliferation, migration, and invasion of gastric cancer BGC-823 cells were significantly inhibited, and the apoptotic rates were significantly increased in the lentivirus-transfected (short hairpin-PRDX6) group. Western blot analysis showed that the expression of Bax protein increased, whereas that of proliferating cell nuclear antigen, Bcl-2, PI3K, phospho (p-Akt), and phosphorylated-mammalian target of rapamycin (mTOR) decreased significantly compared with that in WT and vector groups (P < 0.05). Conclusion: The knockdown of PRDX6 gene expression in BGC-823 cells can inhibit the proliferation, migration, and invasion of gastric cancer cells and promote apoptosis, thereby affecting gastric cancer cells.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Gástricas / Peroxiredoxina VI Límite: Humans Idioma: En Revista: J Cancer Res Ther Asunto de la revista: NEOPLASIAS / TERAPEUTICA Año: 2022 Tipo del documento: Article Pais de publicación: India
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Gástricas / Peroxiredoxina VI Límite: Humans Idioma: En Revista: J Cancer Res Ther Asunto de la revista: NEOPLASIAS / TERAPEUTICA Año: 2022 Tipo del documento: Article Pais de publicación: India