Your browser doesn't support javascript.
loading
A kinetic model for the impact of packaging signal mimics on genome encapsulation.
de Bruijn, René; Wielstra, Pieta Cornelia Martha; Calcines-Cruz, Carlos; van Waveren, Tom; Hernandez-Garcia, Armando; van der Schoot, Paul.
Afiliación
  • de Bruijn R; Department of Applied Physics, Eindhoven University of Technology, Eindhoven, the Netherlands; Institute for Complex Molecular Systems, Eindhoven University of Technology, Eindhoven, the Netherlands. Electronic address: r.a.j.d.bruijn@tue.nl.
  • Wielstra PCM; Department of Applied Physics, Eindhoven University of Technology, Eindhoven, the Netherlands.
  • Calcines-Cruz C; Department of Chemistry of Biomacromolecules, Institute of Chemistry, National Autonomous University of Mexico, Mexico City, Mexico.
  • van Waveren T; Department of Applied Physics, Eindhoven University of Technology, Eindhoven, the Netherlands.
  • Hernandez-Garcia A; Department of Chemistry of Biomacromolecules, Institute of Chemistry, National Autonomous University of Mexico, Mexico City, Mexico.
  • van der Schoot P; Department of Applied Physics, Eindhoven University of Technology, Eindhoven, the Netherlands.
Biophys J ; 121(13): 2583-2599, 2022 07 05.
Article en En | MEDLINE | ID: mdl-35642255
Inspired by recent experiments on the spontaneous assembly of virus-like particles from a solution containing a synthetic coat protein and double-stranded DNA, we put forward a kinetic model that has as main ingredients a stochastic nucleation and a deterministic growth process. The efficiency and rate of DNA packaging strongly increase after tiling the DNA with CRISPR-Cas proteins at predesignated locations, mimicking assembly signals in viruses. Our model shows that treating these proteins as nucleation-inducing diffusion barriers is sufficient to explain the experimentally observed increase in encapsulation efficiency, but only if the nucleation rate is sufficiently high. We find an optimum in the encapsulation kinetics for conditions where the number of packaging signal mimics is equal to the number of nucleation events that can occur during the time required to fully encapsulate the DNA template, presuming that the nucleation events can only take place adjacent to a packaging signal. Our theory is in satisfactory agreement with the available experimental data.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ensamble de Virus / Empaquetamiento del ADN Idioma: En Revista: Biophys J Año: 2022 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ensamble de Virus / Empaquetamiento del ADN Idioma: En Revista: Biophys J Año: 2022 Tipo del documento: Article Pais de publicación: Estados Unidos