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Assessing the Activity of Antimicrobial Peptides Against Common Marine Bacteria Located in Rotifer (Brachionus plicatilis) Cultures.
Woods, Chelsea; Woolley, Lindsey; Partridge, Gavin; Chen, Mengqi; Haney, Evan F; Hancock, Robert E W; Buller, Nicky; Currie, Andrew.
Afiliación
  • Woods C; College of Science, Murdoch University, Engineering & Education, HealthPerth, WA, Australia. cheslea.woodsnielsen@gmail.com.
  • Woolley L; Department of Primary Industries and Regional Development, Fremantle, Perth, WA, 6160, Australia. cheslea.woodsnielsen@gmail.com.
  • Partridge G; College of Science, Murdoch University, Engineering & Education, HealthPerth, WA, Australia.
  • Chen M; Department of Primary Industries and Regional Development, Fremantle, Perth, WA, 6160, Australia.
  • Haney EF; College of Science, Murdoch University, Engineering & Education, HealthPerth, WA, Australia.
  • Hancock REW; Department of Primary Industries and Regional Development, Fremantle, Perth, WA, 6160, Australia.
  • Buller N; Department of Primary Industries and Regional Development, Fremantle, Perth, WA, 6160, Australia.
  • Currie A; Centre for Microbial Diseases and Immunity Research, Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC, Canada.
Probiotics Antimicrob Proteins ; 14(4): 620-629, 2022 08.
Article en En | MEDLINE | ID: mdl-35612776
Rotifers are used as the first feed for marine fish larvae and are grown in large cultures that have high loads of organic matter and heterotrophic bacteria; these bacteria are passed on to the developing fish larvae and can potentially lead to bacterial infections. A modified minimum inhibitory concentration (MIC) protocol for antimicrobial peptides was used to determine the potency of ten antimicrobial peptides (AMPs) in artificial seawater relevant to a rotifer culture (salinity of 25‰) against common marine pathogens. All of the AMPs had antimicrobial activity against the bacterial isolates when the salt concentration was approximately zero. However, in high salt concentrations, the majority of the AMPs had an MIC value greater than 65 µg mL-1 in artificial seawater (25‰). The only exceptions were 2009 (32.5 µg mL-1) and 3002 (32.5 µg mL-1) against Vibrio rotiferianus and Tenacibaculum discolor, respectively. The selected synthetic AMPs were not effective at reducing the bacterial load in brackish salt concentrations of a typical commercial rotifer culture (25‰).
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Rotíferos / Péptidos Antimicrobianos Límite: Animals Idioma: En Revista: Probiotics Antimicrob Proteins Año: 2022 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Rotíferos / Péptidos Antimicrobianos Límite: Animals Idioma: En Revista: Probiotics Antimicrob Proteins Año: 2022 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos