Rodent Lung Tissue Sample Preparation and Processing for Shotgun Proteomics.

Piyadasa, Hadeesha; Lao, Ying; Krokhin, Oleg; Mookherjee, Neeloffer

Piyadasa, Hadeesha; Lao, Ying; Krokhin, Oleg; Mookherjee, Neeloffer.

Afiliación

Piyadasa H; Manitoba Centre for Proteomics and Systems Biology, Department of Internal Medicine, University of Manitoba, Winnipeg, MB, Canada.
Lao Y; Department of Immunology, University of Manitoba, Winnipeg, MB, Canada.
Krokhin O; Department of Pathology, School of Medicine, Stanford University, Palo Alto, CA, USA.
Mookherjee N; Manitoba Centre for Proteomics and Systems Biology, Department of Internal Medicine, University of Manitoba, Winnipeg, MB, Canada.

Methods Mol Biol ; 2456: 53-62, 2022.

Article en En | MEDLINE | ID: mdl-35612734

RESUMEN

Mass spectrometry (MS) is a routinely used approach to characterize global protein profile in various biological samples. Here we describe rodent lung tissue homogenization, sample preparation, and liquid chromatography with tandem mass spectrometry (LC-MS/MS) method for shotgun proteomics.

Asunto(s)

Proteómica; Espectrometría de Masas en Tándem; Animales; Cromatografía Liquida/métodos; Pulmón; Proteómica/métodos; Roedores

Palabras clave

Mass spectrometry; Rodent lungs; Sample preparation; Shotgun proteomics

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteómica / Espectrometría de Masas en Tándem Límite: Animals Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2022 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos

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