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Characterization of a Bacillus cereus strain associated with a large feed-related outbreak of severe infection in pigs.
Calvigioni, Marco; Cara, Alice; Celandroni, Francesco; Mazzantini, Diletta; Panattoni, Adelaide; Tirloni, Erica; Bernardi, Cristian; Pinotti, Luciano; Stella, Simone; Ghelardi, Emilia.
Afiliación
  • Calvigioni M; Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Pisa, Italy.
  • Cara A; Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Pisa, Italy.
  • Celandroni F; Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Pisa, Italy.
  • Mazzantini D; Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Pisa, Italy.
  • Panattoni A; Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Pisa, Italy.
  • Tirloni E; Department of Health, Animal Science and Food Safety, University of Milan, Milan, Italy.
  • Bernardi C; Department of Health, Animal Science and Food Safety, University of Milan, Milan, Italy.
  • Pinotti L; Department of Health, Animal Science and Food Safety, University of Milan, Milan, Italy.
  • Stella S; Department of Health, Animal Science and Food Safety, University of Milan, Milan, Italy.
  • Ghelardi E; Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Pisa, Italy.
J Appl Microbiol ; 133(2): 1078-1088, 2022 Aug.
Article en En | MEDLINE | ID: mdl-35611609
AIMS: Bacillus cereus is often responsible for foodborne diseases and both local and systemic infections in humans. Cases of infection in other mammals are rather rare. In this study, we report a B. cereus feed-related outbreak that caused the death of 6234 pigs in Italy. METHODS AND RESULTS: Massive doses of a Gram-positive, spore-forming bacterium were recovered from the animal feed, faeces of survived pigs and intestinal content of dead ones. The B. cereus MM1 strain was identified by MALDI-TOF MS and typified by RAPD-PCR. The isolate was tested for the production of PC-PLC, proteases, hemolysins and biofilm, for motility, as well as for the presence of genes encoding tissue-degrading enzymes and toxins. Antimicrobial resistance and pathogenicity in Galleria mellonella larvae were also investigated. Our results show that the isolated B. cereus strain is swimming-proficient, produces PC-PLC, proteases, hemolysins, biofilm and carries many virulence genes. The strain shows high pathogenicity in G. mellonella larvae. CONCLUSIONS: The isolated B. cereus strain demonstrates an aggressive profile of pathogenicity and virulence, being able to produce a wide range of determinants potentially hazardous to pigs' health. SIGNIFICANCE AND IMPACT OF STUDY: This study highlights the proficiency of B. cereus to behave as a devastating pathogen in swine if ingested at high doses and underlines that more stringent quality controls are needed for livestock feeds and supplements.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacillus cereus / Infecciones por Bacterias Grampositivas / Proteínas Hemolisinas / Alimentación Animal Tipo de estudio: Risk_factors_studies Límite: Animals Idioma: En Revista: J Appl Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacillus cereus / Infecciones por Bacterias Grampositivas / Proteínas Hemolisinas / Alimentación Animal Tipo de estudio: Risk_factors_studies Límite: Animals Idioma: En Revista: J Appl Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido