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Hyperpolarization-activated cyclic nucleotide-gated 2 contributes to electroacupuncture analgesia on lumbar disc herniation-induced radicular pain through activation of microglia in spinal dorsal horn.
Qingguang, Qin; Zujiang, Chen; Weimin, Fan; Junhua, L I; Liqing, Liao; Yikai, L I.
Afiliación
  • Qingguang Q; School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.
  • Zujiang C; School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.
  • Weimin F; School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.
  • Junhua LI; School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.
  • Liqing L; School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.
  • Yikai LI; School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.
J Tradit Chin Med ; 42(3): 372-378, 2022 06.
Article en En | MEDLINE | ID: mdl-35610006
OBJECTIVE: To explore the mechanisms of dorsal root ganglia and spinal microglia cascade cross in electroacupuncture (EA) analgesia in the treatment of lumbar disc herniation. METHODS: A rat model of lumbar disc herniation (LDH) was established, EA was administered at Huantiao (GB30) acupoint 30 min once a day, for 3 d. Before and after modeling, and after EA, mechanical allodynia thresholds were detected. Hyperpolarization-activated cyclic nucleotide-gated 2 (HCN2) in dorsal root ganglia was detected by quantitative polymerase chain reaction (qPCR) and Western blot. C-X3-C motif chemokine ligand 1 (CX3CL1) and activity of microglia in spinal cord was observed separately qPCR and immunofluorescence staining. RESULTS: The mechanical allodynia threshold of the right planta of model rats was significantly reduced ( < 0.01), EA increased the mechanical pain threshold of rats ( < 0.01), and decreased HCN2 mRNA, and protein expression, reduced the expression of CX3CL1 and the activation of microglia. ZD7288 (a blocker of HCN channel) reduced the analgesic effect of EA from 1.83 ± 0.84 to 0.74 ± 0.20 ( < 0.05), and the expression of CX3CL1 in the spinal cord decreased from 0.52 ± 0.11 to 0.15 ± 0.05 ( < 0.01). CONCLUSION: EA analgesia on the radicular pain of LDH is definite. EA reduced the expression of HCN2 channel in the dorsal root ganglion, thereby decreasing the noxious stimulation entered to microglia in spinal dorsal horn. Our work supports EA is an effective treatment for radicular pain of LDH.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Electroacupuntura / Desplazamiento del Disco Intervertebral / Neuralgia Límite: Animals / Humans Idioma: En Revista: J Tradit Chin Med Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: China
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Electroacupuntura / Desplazamiento del Disco Intervertebral / Neuralgia Límite: Animals / Humans Idioma: En Revista: J Tradit Chin Med Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: China