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Fibrin clot characteristics and anticoagulant response in a SARS-CoV-2-infected endothelial model.
McCafferty, Conor; Lee, Leo; Cai, Tengyi; Praporski, Slavica; Stolper, Julian; Karlaftis, Vasiliki; Attard, Chantal; Myint, David; Carey, Leeanne M; Howells, David W; Donnan, Geoffrey A; Davis, Stephen; Ma, Henry; Crewther, Sheila; Nguyen, Vinh A; Van Den Helm, Suelyn; Letunica, Natasha; Swaney, Ella; Elliott, David; Subbarao, Kanta; Ignjatovic, Vera; Monagle, Paul.
Afiliación
  • McCafferty C; Department of Paediatrics The University of Melbourne Melbourne Victoria Australia.
  • Lee L; Haematology Murdoch Children's Research Institute Melbourne Victoria Australia.
  • Cai T; Department of Microbiology and Immunology The Peter Doherty Institute for Infection and Immunity The University of Melbourne Melbourne Victoria Australia.
  • Praporski S; Department of Paediatrics The University of Melbourne Melbourne Victoria Australia.
  • Stolper J; Haematology Murdoch Children's Research Institute Melbourne Victoria Australia.
  • Karlaftis V; Haematology Murdoch Children's Research Institute Melbourne Victoria Australia.
  • Attard C; Heart Regeneration Murdoch Children's Research Institute Melbourne Victoria Australia.
  • Myint D; Haematology Murdoch Children's Research Institute Melbourne Victoria Australia.
  • Carey LM; Department of Paediatrics The University of Melbourne Melbourne Victoria Australia.
  • Howells DW; Haematology Murdoch Children's Research Institute Melbourne Victoria Australia.
  • Donnan GA; TA Scientific Pty. Ltd. Taren Point Sydney New South Wales Australia.
  • Davis S; Department of Occupational Therapy Social Work and Social Policy La Trobe University Melbourne Victoria Australia.
  • Ma H; Neurorehabilitation and Recovery Florey Institute of Neuroscience and Mental Health University of Melbourne Melbourne Victoria Australia.
  • Crewther S; Tasmanian School of Medicine University of Tasmania Hobart Tasmania Australia.
  • Nguyen VA; Melbourne Brain Centre Royal Melbourne Hospital and University of Melbourne Melbourne Victoria Australia.
  • Van Den Helm S; Melbourne Brain Centre Royal Melbourne Hospital and University of Melbourne Melbourne Victoria Australia.
  • Letunica N; Department of Neurology and Stroke Monash Health Hospital Melbourne Victoria Australia.
  • Swaney E; Department of Psychology and Counselling La Trobe University Melbourne Victoria Australia.
  • Elliott D; Department of Psychology and Counselling La Trobe University Melbourne Victoria Australia.
  • Subbarao K; Haematology Murdoch Children's Research Institute Melbourne Victoria Australia.
  • Ignjatovic V; Haematology Murdoch Children's Research Institute Melbourne Victoria Australia.
  • Monagle P; Department of Paediatrics The University of Melbourne Melbourne Victoria Australia.
EJHaem ; 3(2): 326-334, 2022 May.
Article en En | MEDLINE | ID: mdl-35602246
Coronavirus disease 2019 (COVID-19) patients have increased thrombosis risk. With increasing age, there is an increase in COVID-19 severity. Additionally, adults with a history of vasculopathy have the highest thrombotic risk in COVID-19. The mechanisms of these clinical differences in risk remain unclear. Human umbilical vein endothelial cells (HUVECs) were infected with SARS-CoV-2, influenza A/Singapore/6/86 (H1N1) or mock-infected prior to incubation with plasma from healthy children, healthy adults or vasculopathic adults. Fibrin on surface of cells was observed using scanning electron microscopy, and fibrin characteristics were quantified. This experiment was repeated in the presence of bivalirudin, defibrotide, low-molecular-weight-heparin (LMWH) and unfractionated heparin (UFH). Fibrin formed on SARS-CoV-2 infected HUVECs was densely packed and contained more fibrin compared to mock-infected cells. Fibrin generated from child plasma was the thicker than fibrin generated in vasculopathic adult plasma (p = 0.0165). Clot formation was inhibited by LMWH (0.5 U/ml) and UFH (0.1-0.7 U/ml). We show that in the context of the SARS-CoV-2 infection on an endothelial culture, plasma from vasculopathic adults produces fibrin clots with thinner fibrin, indicating that the plasma coagulation system may play a role in determining the thrombotic outcome of SARS-CoV-2 infection. Heparinoid anticoagulants were most effective at preventing clot formation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: EJHaem Año: 2022 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: EJHaem Año: 2022 Tipo del documento: Article Pais de publicación: Estados Unidos