Your browser doesn't support javascript.
loading
PACT establishes a posttranscriptional brake on mitochondrial biogenesis by promoting the maturation of miR-181c.
Dogan, Asli E; Hamid, Syed M; Yildirim, Asli D; Yildirim, Zehra; Sen, Ganes; Riera, Celine E; Gottlieb, Roberta A; Erbay, Ebru.
Afiliación
  • Dogan AE; Smidt Heart Institute, Cedars-Sinai Medical Center, Los Angeles, California, USA; Department of Molecular Biology and Genetics, National Nanotechnology Center, Bilkent University, Ankara, Turkey.
  • Hamid SM; Smidt Heart Institute, Cedars-Sinai Medical Center, Los Angeles, California, USA.
  • Yildirim AD; Department of Molecular Biology and Genetics, National Nanotechnology Center, Bilkent University, Ankara, Turkey.
  • Yildirim Z; Smidt Heart Institute, Cedars-Sinai Medical Center, Los Angeles, California, USA; Department of Molecular Biology and Genetics, National Nanotechnology Center, Bilkent University, Ankara, Turkey.
  • Sen G; Department of Inflammation and Immunity, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, USA.
  • Riera CE; Department of Biomedical Sciences, Center for Neural Science and Medicine, Cedars-Sinai Medical Center, Los Angeles, California, USA; Department of Neurology, Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, California, USA; David Geffen School of Medicin
  • Gottlieb RA; Department of Cardiology, Smidt Heart Institute, Cedars-Sinai Medical Center, Los Angeles, California, USA.
  • Erbay E; David Geffen School of Medicine, University of California, Los Angeles, California, USA; Department of Cardiology, Smidt Heart Institute, Cedars-Sinai Medical Center, Los Angeles, California, USA. Electronic address: ebru.erbay@ucla.edu.
J Biol Chem ; 298(7): 102050, 2022 07.
Article en En | MEDLINE | ID: mdl-35598827
The double-stranded RNA-dependent protein kinase activating protein (PACT), an RNA-binding protein that is part of the RNA-induced silencing complex, plays a key role in miR-mediated translational repression. Previous studies showed that PACT regulates the expression of various miRs, selects the miR strand to be loaded onto RNA-induced silencing complex, and determines proper miR length. Apart from PACT's role in mediating the antiviral response in immune cells, what PACT does in other cell types is unknown. Strikingly, it has also been shown that cold exposure leads to marked downregulation of PACT protein in mouse brown adipose tissue (BAT), where mitochondrial biogenesis and metabolism play a central role. Here, we show that PACT establishes a posttranscriptional brake on mitochondrial biogenesis (mitobiogenesis) by promoting the maturation of miR-181c, a key suppressor of mitobiogenesis that has been shown to target mitochondrial complex IV subunit I (Mtco1) and sirtuin 1 (Sirt1). Consistently, we found that a partial reduction in PACT expression is sufficient to enhance mitobiogenesis in brown adipocytes in culture as well as during BAT activation in mice. In conclusion, we demonstrate an unexpected role for PACT in the regulation of mitochondrial biogenesis and energetics in cells and BAT.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Biogénesis de Organelos / Tejido Adiposo Pardo / Proteínas de Unión al ARN / MicroARNs / Mitocondrias Límite: Animals Idioma: En Revista: J Biol Chem Año: 2022 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Biogénesis de Organelos / Tejido Adiposo Pardo / Proteínas de Unión al ARN / MicroARNs / Mitocondrias Límite: Animals Idioma: En Revista: J Biol Chem Año: 2022 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Estados Unidos