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Controlled human infection with Neisseria lactamica in late pregnancy to measure horizontal transmission and microbiome changes in mother-neonate pairs: a single-arm interventional pilot study protocol.
Theodosiou, Anastasia A; Laver, Jay R; Dale, Adam P; Cleary, David W; Jones, Christine E; Read, Robert C.
Afiliación
  • Theodosiou AA; Clinical and Experimental Sciences, University of Southampton, Southampton, Hampshire, UK at1u17@soton.ac.uk.
  • Laver JR; Clinical and Experimental Sciences, University of Southampton, Southampton, Hampshire, UK.
  • Dale AP; Clinical and Experimental Sciences, University of Southampton, Southampton, Hampshire, UK.
  • Cleary DW; Clinical and Experimental Sciences, University of Southampton, Southampton, Hampshire, UK.
  • Jones CE; Faculty of Medicine and Institute for Life Sciences, University of Southampton, Southampton, UK.
  • Read RC; Clinical and Experimental Sciences, University of Southampton, Southampton, Hampshire, UK.
BMJ Open ; 12(5): e056081, 2022 05 18.
Article en En | MEDLINE | ID: mdl-35584870
INTRODUCTION: Infant upper respiratory microbiota are derived partly from the maternal respiratory tract, and certain microbiota are associated with altered risk of infections and respiratory disease. Neisseria lactamica is a common pharyngeal commensal in young children and is associated with reduced carriage and invasive disease by Neisseria meningitidis. Nasal inoculation with N. lactamica safely and reproducibly reduces N. meningitidis colonisation in healthy adults. We propose nasal inoculation of pregnant women with N. lactamica, to establish if neonatal pharyngeal colonisation occurs after birth, and to characterise microbiome evolution in mother-infant pairs over 1 month post partum. METHODS AND ANALYSIS: 20 healthy pregnant women will receive nasal inoculation with N. lactamica (wild type strain Y92-1009) at 36-38 weeks gestation. Upper respiratory samples, as well as optional breastmilk, umbilical cord blood and infant venous blood samples, will be collected from mother-infant pairs over 1 month post partum. We will assess safety, N. lactamica colonisation (by targeted PCR) and longitudinal microevolution (by whole genome sequencing), and microbiome evolution (by 16S rRNA gene sequencing). ETHICS AND DISSEMINATION: This study has been approved by the London Central Research Ethics Committee (21/PR/0373). Findings will be published in peer-reviewed open-access journals as soon as possible. TRIAL REGISTRATION NUMBER: NCT04784845.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neisseria lactamica / Microbiota / Neisseria meningitidis Aspecto: Ethics Límite: Adult / Child / Child, preschool / Female / Humans / Infant / Newborn / Pregnancy Idioma: En Revista: BMJ Open Año: 2022 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neisseria lactamica / Microbiota / Neisseria meningitidis Aspecto: Ethics Límite: Adult / Child / Child, preschool / Female / Humans / Infant / Newborn / Pregnancy Idioma: En Revista: BMJ Open Año: 2022 Tipo del documento: Article Pais de publicación: Reino Unido