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Temporal perturbation of histone deacetylase activity reveals a requirement for HDAC1-3 in mesendoderm cell differentiation.
Sinniah, Enakshi; Wu, Zhixuan; Shen, Sophie; Naval-Sanchez, Marina; Chen, Xiaoli; Lim, Junxian; Helfer, Abbigail; Iyer, Abishek; Tng, Jiahui; Lucke, Andrew J; Reid, Robert C; Redd, Meredith A; Nefzger, Christian M; Fairlie, David P; Palpant, Nathan J.
Afiliación
  • Sinniah E; Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD, Australia.
  • Wu Z; Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD, Australia.
  • Shen S; Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD, Australia.
  • Naval-Sanchez M; Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD, Australia.
  • Chen X; Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD, Australia.
  • Lim J; Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD, Australia; ARC Centre of Excellence for Advanced Molecular Imaging, Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD 4072, Australia.
  • Helfer A; Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD, Australia.
  • Iyer A; Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD, Australia; ARC Centre of Excellence for Advanced Molecular Imaging, Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD 4072, Australia.
  • Tng J; Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD, Australia; ARC Centre of Excellence for Advanced Molecular Imaging, Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD 4072, Australia.
  • Lucke AJ; Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD, Australia; ARC Centre of Excellence for Advanced Molecular Imaging, Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD 4072, Australia.
  • Reid RC; Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD, Australia; ARC Centre of Excellence for Advanced Molecular Imaging, Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD 4072, Australia.
  • Redd MA; Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD, Australia.
  • Nefzger CM; Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD, Australia.
  • Fairlie DP; Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD, Australia; ARC Centre of Excellence for Advanced Molecular Imaging, Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD 4072, Australia.
  • Palpant NJ; Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD, Australia. Electronic address: n.palpant@uq.edu.au.
Cell Rep ; 39(7): 110818, 2022 05 17.
Article en En | MEDLINE | ID: mdl-35584683
Histone deacetylases (HDACs) are a class of enzymes that control chromatin state and influence cell fate. We evaluated the chromatin accessibility and transcriptome dynamics of zinc-containing HDACs during cell differentiation in vitro coupled with chemical perturbation to identify the role of HDACs in mesendoderm cell fate specification. Single-cell RNA sequencing analyses of HDAC expression during human pluripotent stem cell (hPSC) differentiation in vitro and mouse gastrulation in vivo reveal a unique association of HDAC1 and -3 with mesendoderm gene programs during exit from pluripotency. Functional perturbation with small molecules reveals that inhibition of HDAC1 and -3, but not HDAC2, induces mesoderm while impeding endoderm and early cardiac progenitor specification. These data identify unique biological functions of the structurally homologous enzymes HDAC1-3 in influencing hPSC differentiation from pluripotency toward mesendodermal and cardiac progenitor populations.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Madre Pluripotentes / Endodermo / Histona Desacetilasas Límite: Animals Idioma: En Revista: Cell Rep Año: 2022 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Madre Pluripotentes / Endodermo / Histona Desacetilasas Límite: Animals Idioma: En Revista: Cell Rep Año: 2022 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos