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Determining the Cytosolic Stability of Small DNA Nanostructures In Cellula.
Mathur, Divita; Rogers, Katherine E; Díaz, Sebastián A; Muroski, Megan E; Klein, William P; Nag, Okhil K; Lee, Kwahun; Field, Lauren D; Delehanty, James B; Medintz, Igor L.
Afiliación
  • Mathur D; College of Science, George Mason University, Fairfax, Virginia 22030, United States.
  • Rogers KE; Center for Bio/Molecular Science and Engineering Code 6900, US Naval Research Laboratory, Washington, DC 20375, United States.
  • Díaz SA; Center for Bio/Molecular Science and Engineering Code 6900, US Naval Research Laboratory, Washington, DC 20375, United States.
  • Muroski ME; Fischell Department of Bioengineering, University of Maryland, College Park, Maryland 20742, United States.
  • Klein WP; Center for Bio/Molecular Science and Engineering Code 6900, US Naval Research Laboratory, Washington, DC 20375, United States.
  • Nag OK; Center for Bio/Molecular Science and Engineering Code 6900, US Naval Research Laboratory, Washington, DC 20375, United States.
  • Lee K; American Society of Engineering Education, Washington, DC 20036, United States.
  • Field LD; Center for Bio/Molecular Science and Engineering Code 6900, US Naval Research Laboratory, Washington, DC 20375, United States.
  • Delehanty JB; National Research Council, Washington, DC 20001, United States.
  • Medintz IL; Center for Bio/Molecular Science and Engineering Code 6900, US Naval Research Laboratory, Washington, DC 20375, United States.
Nano Lett ; 22(12): 5037-5045, 2022 06 22.
Article en En | MEDLINE | ID: mdl-35580267
DNA nanostructures have proven potential in biomedicine. However, their intracellular interactions─especially cytosolic stability─remain mostly unknown and attempts to discern this are confounded by the complexities of endocytic uptake and entrapment. Here, we bypass the endocytic uptake and evaluate the DNA structural stability directly in live cells. Commonly used DNA structures─crosshairs and a tetrahedron─were labeled with a multistep Förster resonance energy transfer dye cascade and microinjected into the cytosol of transformed and primary cells. Energy transfer loss, as monitored by fluorescence microscopy, reported the structure's direct time-resolved breakdown in cellula. The results showed rapid degradation of the DNA crosshair within 20 min, while the tetrahedron remained consistently intact for at least 1 h postinjection. Nuclease assays in conjunction with a current understanding of the tetrahedron's torsional rigidity confirmed its higher stability. Such studies can inform design parameters for future DNA nanostructures where programmable degradation rates may be required.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nanoestructuras Idioma: En Revista: Nano Lett Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nanoestructuras Idioma: En Revista: Nano Lett Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos