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An efficient immunoassay for the B cell help function of SARS-CoV-2-specific memory CD4+ T cells.
Ansari, Asgar; Sachan, Shilpa; Jit, Bimal Prasad; Sharma, Ashok; Coshic, Poonam; Sette, Alessandro; Weiskopf, Daniela; Gupta, Nimesh.
Afiliación
  • Ansari A; Vaccine Immunology Laboratory, National Institute of Immunology, New Delhi 110067, India.
  • Sachan S; Vaccine Immunology Laboratory, National Institute of Immunology, New Delhi 110067, India.
  • Jit BP; Department of Biochemistry, All India Institute of Medical Sciences, New Delhi 110029, India.
  • Sharma A; Department of Biochemistry, All India Institute of Medical Sciences, New Delhi 110029, India.
  • Coshic P; Department of Transfusion Medicine, All India Institute of Medical Sciences, New Delhi 110029, India.
  • Sette A; Center for Infectious Disease and Vaccine Research, La Jolla Institute for Immunology, La Jolla, CA 92037, USA.
  • Weiskopf D; Department of Medicine, Division of Infectious Diseases and Global Public Health, University of California, San Diego, La Jolla, CA 92037, USA.
  • Gupta N; Center for Infectious Disease and Vaccine Research, La Jolla Institute for Immunology, La Jolla, CA 92037, USA.
Cell Rep Methods ; 2(6): 100224, 2022 06 20.
Article en En | MEDLINE | ID: mdl-35571764
The B cell "help" function of CD4+ T cells is an important mechanism of adaptive immunity. Here, we describe improved antigen-specific T-B cocultures for quantitative measurement of T cell-dependent B cell responses, with as few as ∼90 T cells. Utilizing M. tuberculosis (Mtb), we show that early priming and activation of CD4+ T cells is important for productive interaction between T and B cells and that similar effects are achieved by supplementing cocultures with monocytes. We find that monocytes promote survivability of B cells via BAFF and stem cell growth factor (SCGF)/C-type lectin domain family 11 member A (CLEC11A), but this alone does not fully recapitulate the effects of monocyte supplementation. Importantly, we demonstrate improved activation and immunological output of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-specific memory CD4+ T-B cell cocultures with the inclusion of monocytes. This method may therefore provide a more sensitive assay to evaluate the B cell help quality of memory CD4+ T cells, for example, after vaccination or natural infection.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Linfocitos B / Linfocitos T CD4-Positivos / COVID-19 / Memoria Inmunológica Límite: Humans Idioma: En Revista: Cell Rep Methods Año: 2022 Tipo del documento: Article País de afiliación: India Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Linfocitos B / Linfocitos T CD4-Positivos / COVID-19 / Memoria Inmunológica Límite: Humans Idioma: En Revista: Cell Rep Methods Año: 2022 Tipo del documento: Article País de afiliación: India Pais de publicación: Estados Unidos