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Efficient recovery of attenuated canine distemper virus from cDNA.
Wyss, Marianne; Gradauskaite, Vaiva; Ebert, Nadine; Thiel, Volker; Zurbriggen, Andreas; Plattet, Philippe.
Afiliación
  • Wyss M; Division of Neurological Sciences, Vetsuisse Faculty, University of Bern, Switzerland.
  • Gradauskaite V; Division of Neurological Sciences, Vetsuisse Faculty, University of Bern, Switzerland; Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland.
  • Ebert N; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland; Institute of Virology and Immunology, Mittelhäusern, Bern, Switzerland.
  • Thiel V; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland; Institute of Virology and Immunology, Mittelhäusern, Bern, Switzerland.
  • Zurbriggen A; Division of Neurological Sciences, Vetsuisse Faculty, University of Bern, Switzerland.
  • Plattet P; Division of Neurological Sciences, Vetsuisse Faculty, University of Bern, Switzerland. Electronic address: philippe.plattet@vetsuisse.unibe.ch.
Virus Res ; 316: 198796, 2022 07 15.
Article en En | MEDLINE | ID: mdl-35568090
To provide insights into the biology of the attenuated canine distemper virus (CDV) Onderstepoort (OP) strain (large plaque forming variant), design next-generation multivalent vaccines, or further investigate its promising potential as an oncolytic vector, we employed contemporary modifications to establish an efficient OP-CDV-based reverse genetics platform. Successful viral rescue was obtained however only upon recovery of a completely conserved charged residue (V13E) residing at the N-terminal region of the large protein (L). Although L-V13 and L-V13E did not display drastic differences in cellular localization and physical interaction with P, efficient polymerase complex (P+ L) activity was recorded only with L-V13E. Interestingly, grafting mNeonGreen to the viral N protein via a P2A ribosomal skipping sequence (OPneon) and its derivative V-protein-knockout variant (OPneon-Vko) exhibited delayed replication kinetics in cultured cells. Collectively, we established an efficient OP-CDV-based reverse genetics system that enables the design of various strategies potentially contributing to veterinary medicine and research.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Moquillo / Virus del Moquillo Canino Límite: Animals Idioma: En Revista: Virus Res Asunto de la revista: VIROLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Países Bajos
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Moquillo / Virus del Moquillo Canino Límite: Animals Idioma: En Revista: Virus Res Asunto de la revista: VIROLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Países Bajos