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Amino acid sequence of two new milk-clotting proteases from the macroalga Gracilaria edulis.
Arbita, Ariestya Arlene; Paul, Nicholas A; Cox, Julian; Zhao, Jian.
Afiliación
  • Arbita AA; School of Chemical Engineering, The University of New South Wales, Sydney, NSW 2052, Australia; School of Chemical Engineering, Faculty of Industrial and Technology, Parahyangan Catholic University, Ciumbuleuit 94, Bandung 40141, Indonesia.
  • Paul NA; School of Science and Engineering, University of the Sunshine Coast, Maroochydore, Queensland 4558, Australia.
  • Cox J; School of Chemical Engineering, The University of New South Wales, Sydney, NSW 2052, Australia.
  • Zhao J; School of Chemical Engineering, The University of New South Wales, Sydney, NSW 2052, Australia. Electronic address: jian.zhao@unsw.edu.au.
Int J Biol Macromol ; 211: 499-505, 2022 Jun 30.
Article en En | MEDLINE | ID: mdl-35561860
This study is aimed at identifying and characterising the proteases we previously extracted from the red seaweed Gracilaria edulis with the potential as milk-clotting enzymes. The protease extract was first analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and zymography. Two protease bands with a molecular weight of 44 and 108 kDa were identified, and analysed using in-gel digestion and liquid chromatography-tandem mass spectrometry/mass spectrometry (LC-MS/MS). Eight peptides from the LC-MS/MS analysis matched those in existing protein databases but they were not related to any protease of the genera Gracilaria and Hydropuntia. Further analysis revealed that more than 80% of the peptide sequence of the algal proteases matched with those from members of the bacteria kingdom, including Gallaecimonas and Alteromonas. Among these, twelve matching homolog proteases were identified as metalloprotease and serine protease. The results indicated that the algal proteases have a close relationship with both algae and bacteria, and suggest that the proteases might have resulted from past bacterial colonisation of the algae and subsequent horizontal gene transfer between bacteria and algae.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Algas Marinas / Gracilaria Límite: Animals Idioma: En Revista: Int J Biol Macromol Año: 2022 Tipo del documento: Article País de afiliación: Indonesia Pais de publicación: Países Bajos
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Algas Marinas / Gracilaria Límite: Animals Idioma: En Revista: Int J Biol Macromol Año: 2022 Tipo del documento: Article País de afiliación: Indonesia Pais de publicación: Países Bajos