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Estimating the two graph dextran-stearic acid-spermine polymers based on iron oxide nanoparticles as carrier for gene delivery.
Kazemi-Ashtiyani, Mehrnoosh; Hajipour-Verdom, Behnam; Satari, Mohammad; Abdolmaleki, Parviz; Hosseinkhani, Saman; Shaki, Hossein.
Afiliación
  • Kazemi-Ashtiyani M; Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
  • Hajipour-Verdom B; Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
  • Satari M; Department of Biology, Faculty of Sciences, Malayer University, Malayer, Iran.
  • Abdolmaleki P; Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
  • Hosseinkhani S; Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
  • Shaki H; Biomedical Engineering Division, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran.
Biopolymers ; 113(7): e23491, 2022 Jul.
Article en En | MEDLINE | ID: mdl-35560028
Non-viral gene carriers have shown noticeable potential in gene delivery because of limited side effects, biocompatibility, simplicity, and the ability to take advantage of electrostatic interactions. However, the low transfection rate of non-viral vectors under physiological conditions is controversial. This study aimed to decrease the transfection time using a static magnetic field. We used self-assembled cationic polysaccharides based on dextran-stearic acid-spermine (DSASP) conjugates associated with Fe3 O4 superparamagnetic nanoparticles to investigate their potential as gene carriers to promote the target delivery. Our findings illustrate that the magnetic nanoparticles are spherical with a positive surface charge and exhibit superparamagnetic behavior. The DSASP-pDNA/Fe3 O4 complexes offered a strong pDNA condensation, protection against DNase degradation, and significant cell viability in HEK 293T cells. Our results demonstrated that although conjugation of stearic acid could play a role in transfection efficiency, DSASP magnetic carriers with more spermine derivatives showed better affinity between the amphiphilic polymer and the negatively charged cell membrane.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espermina / Nanopartículas Idioma: En Revista: Biopolymers Año: 2022 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Estados Unidos
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espermina / Nanopartículas Idioma: En Revista: Biopolymers Año: 2022 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Estados Unidos