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Shortening the duration between ovarian removal and cryopreservation helps preserve fertility and maintain ovarian reserve after transplantation in mice.
Okamoto, Naoki; Nakajima, Mariko; Sugishita, Yodo; Takae, Seido; Horage, Yuki; Suzuki, Nao.
Afiliación
  • Okamoto N; Department of Obstetrics and Gynecology, International University of Health and Welfare School of Medicine, Chiba, Japan; Department of Obstetrics and Gynecology, St. Marianna University School of Medicine, Kanagawa, Japan.
  • Nakajima M; Department of Obstetrics and Gynecology, St. Marianna University School of Medicine, Kanagawa, Japan.
  • Sugishita Y; Department of Obstetrics and Gynecology, St. Marianna University School of Medicine, Kanagawa, Japan.
  • Takae S; Department of Obstetrics and Gynecology, St. Marianna University School of Medicine, Kanagawa, Japan.
  • Horage Y; Department of Obstetrics and Gynecology, St. Marianna University School of Medicine, Kanagawa, Japan.
  • Suzuki N; Department of Obstetrics and Gynecology, St. Marianna University School of Medicine, Kanagawa, Japan. Electronic address: nao@marianna-u.ac.jp.
F S Sci ; 3(2): 140-147, 2022 05.
Article en En | MEDLINE | ID: mdl-35560011
OBJECTIVE: To evaluate the effect of storage of ovaries before cryopreservation on long-term fertility and ovarian reserve after transplantation in mice. DESIGN: Experimental study. SETTING: University hospital. ANIMAL(S): C57BL/6J and C57BL/6J-Tg (CAG-GFP) female mice. INTERVENTION(S): Storage and cryopreservation of mouse ovaries. Long-term fertility analysis of mice transplanted with thawed ovaries. MAIN OUTCOME MEASURE(S): Estrous cycles, number of live births, ovarian weight, and follicular counts of ovarian grafts. RESULT(S): At the first mating 3 months after ovarian transplantation, the mean number of live births was 2.6 ± 0.6 in the control group (no storage); in the storage groups, the mean number of live births was 2.9 ± 0.7 after 4 hours, 1.3 ± 0.5 after 8 hours, 0.2 ± 0.2 after 12 hours, and 0.8 ± 0.5 after 24 hours of storage; the difference from the control group was significant in the 12-hour storage group. At the second mating 6 months after ovarian transplantation, the mean number of live births was 1.8 ± 0.6 in the control group and 2.4 ± 0.6 and 0.3 ± 0.2 in the 4- and 8-hour storage groups, respectively; no live births occurred in the 12- and 24-hour storage groups. Seven months after ovarian transplantation, the numbers of primordial, primary, early secondary, and late secondary follicles were significantly lower in the 8-, 12-, and 24-hour storage groups than in the control group. CONCLUSION(S): In mice, shortening the storage time of ovaries before cryopreservation preserved fertility and ovarian reserve after transplantation, indicating that human ovaries might be cryopreserved immediately after harvesting or transported as quickly as possible to a cryopreservation facility to allow young patients with cancer to preserve long-term fertility and ovarian reserve.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ovario / Preservación de la Fertilidad / Reserva Ovárica Límite: Animals / Female / Humans Idioma: En Revista: F S Sci Año: 2022 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ovario / Preservación de la Fertilidad / Reserva Ovárica Límite: Animals / Female / Humans Idioma: En Revista: F S Sci Año: 2022 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos