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Anticancer L-Asparaginase and Phytoactive Compounds From Plant Solanum nigrum Against MDR (Methicillindrug resistant) Staphylococcus aureus and Fungal Isolates.
Aisha, Ambreen; Zahra, Saba; Tahir, Imtiaz M; Hussain, Asim; Bano, Naheed; Roobi, Alishbah; Afsheen, Nadia; Saleem, Yasir.
Afiliación
  • Aisha A; Department of Biochemistry, Faisalabad Medical University, Faisalabad, Pakistan.
  • Zahra S; College of Marine Life Sciences, Ocean University of China, Qingdao, China.
  • Tahir IM; College of Allied Health Professionals, Government College University, Faisalabad, Pakistan.
  • Hussain A; Department of Biochemistry, University of Agriculture Faisalabad, Faisalabad, Pakistan.
  • Bano N; Department of Fisheries & Aquaculture, MNS-University of Agriculture, Multan, Pakistan.
  • Roobi A; Institute of Physiology and Pharmacology, University of Agriculture Faisalabad, Faisalabad, Pakistan.
  • Afsheen N; Department of Biochemistry, Riphah International University, Faisalabad, Pakistan.
  • Saleem Y; Pakistan Council of Scientific and Industrial Research (PCSIR) Labs, Lahore, Pakistan.
Dose Response ; 20(2): 15593258221092379, 2022.
Article en En | MEDLINE | ID: mdl-35558870
L-asparaginase is used in chemotherapy for acute lymphoblastic leukemia and other cancers. L-asparaginase derived from bacterial source triggers immune responses. The current study investigates Solanum nigrum as a novel and latent source of L-asparaginase to minimize immunological reactions. The antitumor activity of SN methanol extract was determined using the potato disc assay. InterPro Chimera and InterPro were used to predict the amino acid sequence of L-asparaginase and its anticancer activity. Purification of the enzyme was carried out to homogeneity of 1.51-fold with a recovery of 61.99%. At optimal conditions of 36.5°C, pH 8.6, and 8.5 g/mL substrate, fruit (crude extract) revealed an L-asparaginase titer of 48.23 U/mL. The molecular weight of the enzyme was calculated to be 32 ± 5 kDa using SDS PAGE. The fruit's total flavonoids and phenolic contents are 0.42 ± .030 g/mL and 94 ± 1.9 mg CAE, respectively. Anti-tumorigenic efficacy was determined to be 66% against Agrobacterium tumefaciens. Additionally, the extract possesses potent antifungal and antibacterial properties. Molecular docking provided the structural motifs and underlying interactions between L-asparaginase, N-acetylglucosamine, murine, and chitin. SN contains high levels of the enzyme L-asparaginase and phytochemicals, making it a potential source of anticancer drugs.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Dose Response Año: 2022 Tipo del documento: Article País de afiliación: Pakistán Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Dose Response Año: 2022 Tipo del documento: Article País de afiliación: Pakistán Pais de publicación: Estados Unidos