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Evaluation of the tumoricidal efficacy of adoptive cell transfer using hepatocellular carcinoma-derived organoids.
Zhou, Zizhen; Yan, Xiaoluan; Shi, Wanwan; Tan, Kangan; Shao, Chen; Wang, Yan; Wang, Guiqiang; Hong, Yuan.
Afiliación
  • Zhou Z; Infectious Diseases Department, Peking University First Hospital, Beijing, China.
  • Yan X; General Surgery Department, Beijing Cancer Hospital, Beijing, China.
  • Shi W; Infectious Diseases Department, Peking University First Hospital, Beijing, China.
  • Tan K; Infectious Diseases Department, Peking University First Hospital, Beijing, China.
  • Shao C; Pathology Department, Capital Medical University Youan Hospital, Beijing, China.
  • Wang Y; Infectious Diseases Department, Peking University First Hospital, Beijing, China.
  • Wang G; Infectious Diseases Department, Peking University First Hospital, Beijing, China.
  • Hong Y; Infectious Diseases Department, Peking University First Hospital, Beijing, China.
J Gastrointest Oncol ; 13(2): 732-743, 2022 Apr.
Article en En | MEDLINE | ID: mdl-35557574
Background: Tumor-derived organoid, namely tumoroid, can realistically retain the clinicopathologic features of original tumors even after long-term in vitro expansion. Here we develop this production methodology derived from hepatocellular carcinoma primary samples and generate a platform to evaluate the tumoricidal efficacy of autologous adoptive cell transfer including tumor infiltrating lymphocytes and peripheral blood lymphocytes. Methods: Haematoxylin and eosin together with immunohistochemistry staining were employed to ascertain the morphologic and histological features of tumoroids and original tumors. Tumor killing ability of T cells was detected by lactate dehydrogenase assay and propidium iodide staining. In tumoroid xenograft mouse model, tumor volumes were measured and T cell functions were examined by flow cytometry technique. Results: Four tumoroids with characteristics of poor differentiation and mild fibrosis were successfully established from fourteen hepatocellular carcinoma samples. More robust antitumor potential and hyper-functional phenotype of all four tumor infiltrating lymphocytes were observed compared to matched peripheral blood lymphocytes in coculture system. In tumoroid xenograft mouse models, however, only one patient-derived tumor infiltrating lymphocytes with the highest antitumor activity can bestow efficient tumor eradication. Conclusions: Hepatocellular carcinoma tumoroid-based models could represent invaluable resources for evaluating the tumoricidal efficacy of autologous adoptive cell transfer. Tumor infiltrating lymphocytes should be a promising and yet-to-be-developed regimen to treat hepatocellular carcinoma.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: J Gastrointest Oncol Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: China
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: J Gastrointest Oncol Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: China