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Patatin-like phospholipase CapV in Escherichia coli - morphological and physiological effects of one amino acid substitution.
Li, Fengyang; Cao, Lianying; Bähre, Heike; Kim, Soo-Kyoung; Schroeder, Kristen; Jonas, Kristina; Koonce, Kira; Mekonnen, Solomon A; Mohanty, Soumitra; Bai, Fengwu; Brauner, Annelie; Lee, Vincent T; Rohde, Manfred; Römling, Ute.
Afiliación
  • Li F; Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, 17177, Stockholm, Sweden. fylee1987@outlook.com.
  • Cao L; College of Veterinary Medicine, Jilin University, Changchun, China. fylee1987@outlook.com.
  • Bähre H; Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, 17177, Stockholm, Sweden.
  • Kim SK; Research Core Unit Metabolomics, Hannover Medical School, Hannover, Germany.
  • Schroeder K; Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD, 20742, USA.
  • Jonas K; Science for Life Laboratory, Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Stockholm, Sweden.
  • Koonce K; Science for Life Laboratory, Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Stockholm, Sweden.
  • Mekonnen SA; Science for Life Laboratory, Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Stockholm, Sweden.
  • Mohanty S; Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD, 20742, USA.
  • Bai F; Department of Microbiology, Tumor and Cell Biology, Division of Clinical Microbiology, Karolinska Institutet and Karolinska University Hospital, 17176, Stockholm, Sweden.
  • Brauner A; School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 200240, Shanghai, China.
  • Lee VT; Department of Microbiology, Tumor and Cell Biology, Division of Clinical Microbiology, Karolinska Institutet and Karolinska University Hospital, 17176, Stockholm, Sweden.
  • Rohde M; Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD, 20742, USA.
  • Römling U; Central Facility for Microscopy, Helmholtz Center for Infection Research, Braunschweig, Germany.
NPJ Biofilms Microbiomes ; 8(1): 39, 2022 05 11.
Article en En | MEDLINE | ID: mdl-35546554
In rod-shaped bacteria, morphological plasticity occurs in response to stress, which blocks cell division to promote filamentation. We demonstrate here that overexpression of the patatin-like phospholipase variant CapVQ329R, but not CapV, causes pronounced sulA-independent pyridoxine-inhibited cell filamentation in the Escherichia coli K-12-derivative MG1655 associated with restriction of flagella production and swimming motility. Conserved amino acids in canonical patatin-like phospholipase A motifs, but not the nucleophilic serine, are required to mediate CapVQ329R phenotypes. Furthermore, CapVQ329R production substantially alters the lipidome and colony morphotype including rdar biofilm formation with modulation of the production of the biofilm activator CsgD, and affects additional bacterial traits such as the efficiency of phage infection and antimicrobial susceptibility. Moreover, genetically diverse commensal and pathogenic E. coli strains and Salmonella typhimurium responded with cell filamentation and modulation in colony morphotype formation to CapVQ329R expression. In conclusion, this work identifies the CapV variant CapVQ329R as a pleiotropic regulator, emphasizes a scaffold function for patatin-like phospholipases, and highlights the impact of the substitution of a single conserved amino acid for protein functionality and alteration of host physiology.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Escherichia coli K12 / Escherichia coli Idioma: En Revista: NPJ Biofilms Microbiomes Año: 2022 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Estados Unidos
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Escherichia coli K12 / Escherichia coli Idioma: En Revista: NPJ Biofilms Microbiomes Año: 2022 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Estados Unidos