Quorum sensing, the communication between microorganisms, is mediated by specific diffusible signal molecules. Adsorption is an important process that influences the transport, transformation and bioavailability of N-acyl homoserine lactone (AHL) in complex natural environments such as soil. To examine the adsorption characteristics of N-hexanoyl, N-octanoyl, N-decanoyl and N-dodecanoyl homoserine lactones in soil, equilibrium and kinetic experiments were conducted in two types of soils (oxisol and alfisol) and monitored using Fourier-transform infrared spectroscopy (FTIR). A pseudo-second-order equation accurately described the sorption kinetics of AHLs in the two soils (R 2 ≥ 0.97, NSD ≤ 21.25%). The AHL sorption reached equilibrium within 24 h and 12 h for oxisol and alfisol, respectively. The sorption kinetics of AHLs adsorbed on the soils were fitted to the Boyd model, suggesting that film diffusion was the rate-limiting process. Partition played a more vital role than surface adsorption in the AHL adsorption process. The adsorption isotherms of AHLs could be described by the Langmuir and Freundlich equation (R 2 ≥ 0.98), indicating that the sorption process involved monolayer sorption and heterogeneous energetic distribution of active sites on the surfaces of the soils. The thermodynamic parameter, Gibbs free energy (ΔG), and a dimensionless parameter showed that the sorption of AHLs was mainly dominated by physical adsorption. Additionally, according to the FTIR data, the electrostatic forces and hydrogen bonding possibly influenced the adsorption of AHLs on the above mentioned two soils. The sorption characteristics of AHLs in soils correlated well with the molecular structure, solubility speciation and log P (n-octanol/water partition coefficient) of AHLs.