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α-2,6-sialic acid/IgG anti-dsDNA ratios correlate with human lupus disease activity and possible mechanisms: A pilot study.
Liou, Lieh-Bang; Wang, Ting-Yi; Liu, I-Ju; Wu, Han-Chung; Ke, Po-Yuan; Fang, Yao-Fan; Chen, Yen-Fu.
Afiliación
  • Liou LB; Division of Rheumatology, Allergy and Immunology, New Taipei Municipal Tucheng Hospital, New Taipei City, Taiwan.
  • Wang TY; Division of Rheumatology, Allergy and Immunology, Chang Gung Memorial Hospital at Linkou, Taoyuan, Taiwan.
  • Liu IJ; School of Medicine, 56081College of Medicine, Chang Gung University, Taoyuan, Taiwan.
  • Wu HC; Division of Rheumatology, Allergy and Immunology, Chang Gung Memorial Hospital at Linkou, Taoyuan, Taiwan.
  • Ke PY; Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan.
  • Fang YF; Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan.
  • Chen YF; Department of Biochemistry & Molecular Biology, College of Medicine, 56081Chang Gung University, Taoyuan, Taiwan.
Lupus ; 31(8): 927-938, 2022 Jul.
Article en En | MEDLINE | ID: mdl-35531921
OBJECTIVE: To study the association of α2,6-sialic acid (SIA) content in serum IgG anti-dsDNA with human systemic lupus erythematosus disease activity index (SLEDAI) and the effect of sialylated and desialylated (deSIA) IgG anti-dsDNA on lupus B cells. METHODS: Blood from lupus patients was collected to determine the ratio of SIA in isolated IgG anti-dsDNA over serum IgG anti-dsDNA (SIA/IgG anti-dsDNA) ratios, which were plotted against SLEDAI using a receiver-operating-characteristics curve. Lupus B cells were cultured in vitro with chimeric sialylated IgG anti-dsDNA and its deSIA form. Culture supernatants were assayed for anti-inflammatory IL-10 and SIA/IgG anti-dsDNA ratios, which were compared among different pre-treatment groups using t-tests. RESULTS: The area-under-the-curve (AUC) for anti-dsDNA levels against SLEDAI was 0.791 positively (95% confidence interval [C.I.]: 0.699-0.884) and SIA/IgG anti-dsDNA ratios against SLEDAI yielded an AUC of 0.705 inversely (95% C.I: 0.601-0.809): not significantly different. SIA/IgG anti-dsDNA ratios discriminated significantly between patients without and patients with proteinuria (p = .046). SIA/IgG anti-dsDNA ratios correlated significantly and positively with serum C3c and C4 levels. Pre-treatment with IgG anti-dsDNA and its immune complexes (dsDNA/IgG anti-dsDNA IC) induced higher IL-10 from lupus B cells than medium pre-treatment (most p < .01 from day 2 to day 5 culture). DeSIA IgG anti-dsDNA IC induced lower IL-10 (p < .05) and lower SIA/IgG anti-dsDNA ratios (p < .001) from lupus B cells than medium and dsDNA pre-treatment. CONCLUSION: α2,6-SIA/IgG anti-dsDNA ratios inversely forecasted SLEDAI scores. Possible mechanisms may be due to the different effects of sialylated and deSIA IgG anti-dsDNA on lupus B cells in terms of IL-10 secretion and SIA/IgG anti-dsDNA ratios.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ácido N-Acetilneuramínico / Lupus Eritematoso Sistémico Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Lupus Asunto de la revista: REUMATOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ácido N-Acetilneuramínico / Lupus Eritematoso Sistémico Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Lupus Asunto de la revista: REUMATOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Reino Unido