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Preparation of functionalized magnetic nanoparticles conjugated with feroxamine and their evaluation for pathogen detection.
Martínez-Matamoros, Diana; Castro-García, Socorro; Balado, Miguel; Matamoros-Veloza, Adriana; Camargo-Valero, Miller Alonso; Cespedes, Oscar; Rodríguez, Jaime; Lemos, Manuel L; Jiménez, Carlos.
Afiliación
  • Martínez-Matamoros D; Centro de Investigacións Científicas Avanzadas (CICA), Departamento de Química, Facultade de Ciencias, Universidade da Coruña 15071 A Coruña Spain jaime.rodriguez@udc.es carlos.jimenez@udc.es.
  • Castro-García S; Centro de Investigacións Científicas Avanzadas (CICA), Departamento de Química, Facultade de Ciencias, Universidade da Coruña 15071 A Coruña Spain jaime.rodriguez@udc.es carlos.jimenez@udc.es.
  • Balado M; Department of Microbiology and Parasitology, Institute of Aquaculture, Universidade de Santiago de Compostela, Campus Sur Santiago de Compostela 15782 Spain.
  • Matamoros-Veloza A; Institute of Functional Surfaces, School of Mechanical Engineering, University of Leeds Leeds LS2 2JT UK.
  • Camargo-Valero MA; BioResource Systems Research Group, School of Civil Engineering, University of Leeds Leeds LS2 9JT UK.
  • Cespedes O; Departamento de Ingeniería Química, Universidad Nacional de Colombia, Campus La Nubia Manizales Colombia.
  • Rodríguez J; Faculty of Mathematics and Physical Sciences, School of Physics and Astronomy, University of Leeds Leeds LS2 9JT UK.
  • Lemos ML; Centro de Investigacións Científicas Avanzadas (CICA), Departamento de Química, Facultade de Ciencias, Universidade da Coruña 15071 A Coruña Spain jaime.rodriguez@udc.es carlos.jimenez@udc.es.
  • Jiménez C; Department of Microbiology and Parasitology, Institute of Aquaculture, Universidade de Santiago de Compostela, Campus Sur Santiago de Compostela 15782 Spain.
RSC Adv ; 9(24): 13533-13542, 2019 Apr 30.
Article en En | MEDLINE | ID: mdl-35519600
This work reports the preparation of a conjugate between amino-functionalized silica magnetite and the siderophore feroxamine. The morphology and properties of the conjugate and intermediate magnetic nanoparticles (MNPs) were examined by powder X-ray diffraction (XRD), Fourier Transform Infrared spectroscopy (FT-IR), Raman spectroscopy, X-ray photoelectron spectroscopy (XPS), magnetization studies, zeta potential measurements, Transmission Electron Microscopy (TEM) and Energy Dispersive X-ray (EDX) mapping. Furthermore, this study investigated the interaction between the functionalized magnetic NPs and Yersinia enterocolitica wild type (WC-A) using Scanning Electron Microscopy (SEM) and TEM images. In addition, the interaction between MNPs and a Y. enterocolitica mutant strain lacking feroxamine receptor FoxA, was also used to study the binding specificity. The results showed that the capture and isolation of Y. enterocolitica by the MNPs took place in all cases. Moreover, the specific interaction between the MNP conjugate and bacteria did not increase after blocking the free amine groups with t-butoxycarbonyl (Boc) and carboxylic acid (COOH) functional groups. Electrostatic surface interactions instead of molecular recognition between MNP conjugate and feroxamine receptor seem to rule the attachment of bacteria to the conjugate.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies Idioma: En Revista: RSC Adv Año: 2019 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies Idioma: En Revista: RSC Adv Año: 2019 Tipo del documento: Article Pais de publicación: Reino Unido