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Grafting of anti-nucleolin aptamer into preformed and remotely loaded liposomes through aptamer-cholesterol post-insertion.
Nsairat, Hamdi; Mahmoud, Ismail S; Odeh, Fadwa; Abuarqoub, Duaa; Al-Azzawi, Hafsa; Zaza, Rand; Qadri, Malak I; Ismail, Said; Al Bawab, Abeer; Awidi, Abdalla; Alshaer, Walhan.
Afiliación
  • Nsairat H; Department of Chemistry, The University of Jordan Amman 11942 Jordan hammdi2000@yahoo.com +962 796167327.
  • Mahmoud IS; Pharmacological and Diagnostic Research Center, Faculty of Pharmacy, Al-Ahliyya Amman University Amman 19328 Jordan.
  • Odeh F; Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences, The Hashemite University Zarqa 13133 Jordan.
  • Abuarqoub D; Department of Chemistry, The University of Jordan Amman 11942 Jordan hammdi2000@yahoo.com +962 796167327.
  • Al-Azzawi H; HMCSR, The University of Jordan Amman 11942 Jordan.
  • Zaza R; Department of Pharmacology and Biomedical Sciences, Faculty of Pharmacy and Medical Sciences, University of Petra Amman 11180 Jordan.
  • Qadri MI; Cell Therapy Center, The University of Jordan Amman 11942 Jordan walhan.alshaer@ju.edu.jo +962 65355000 Ext. 23960 +962 790823678.
  • Ismail S; Cell Therapy Center, The University of Jordan Amman 11942 Jordan walhan.alshaer@ju.edu.jo +962 65355000 Ext. 23960 +962 790823678.
  • Al Bawab A; Cell Therapy Center, The University of Jordan Amman 11942 Jordan walhan.alshaer@ju.edu.jo +962 65355000 Ext. 23960 +962 790823678.
  • Awidi A; Department of Chemistry, The University of Jordan Amman 11942 Jordan hammdi2000@yahoo.com +962 796167327.
  • Alshaer W; Faculty of Medicine, The University of Jordan Amman 11942 Jordan.
RSC Adv ; 10(59): 36219-36229, 2020 Sep 28.
Article en En | MEDLINE | ID: mdl-35517091
A new combination strategy of an active loading and active targeting approach was applied in this work. The liposomes actively loaded with Curcumin (CRM) (LipCRM) were decorated with cholesterol tagged-anti-nucleolin AS1411 aptamer (NCL) via a new post-insertion approach, utilizing the cholesterol as a wedge to incorporate aptamer into the surface of the liposome bilayer. A successful NCL post-insertion was verified by agarose gel electrophoresis and dynamic light scattering (DLS). The cellular uptake of AptNCL-Lip was investigated using flow cytometry and Confocal Laser Scanning Microscopy (CLSM) on two different human breast cancer cell lines (MCF-7 and MDA-MB-231). The uptake and cytotoxicity of loaded CRM were investigated using flow cytometry and MTT assay. Our results showed successful post insertion of NCL aptamer to the surface of Lip. Also, higher cellular uptake was noted for AptNCL-Alexa-LipRhod compared to blank LipRhod in both cell lines. Moreover, CLSM showed prominent endocytosis and uptake of AptNCL-Alexa-LipRhod into the cytoplasm of breast cancer cells. Furthermore, the results showed a significant increase in the uptake and cytotoxicity of AptNCL-LipCRM compared to LipCRM in both cell lines. Overall, our results demonstrate a successful post-insertion of cholesterol-tagged aptamer into liposomes and the possible combination between active loading and active targeting.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: RSC Adv Año: 2020 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: RSC Adv Año: 2020 Tipo del documento: Article Pais de publicación: Reino Unido