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Helicobacter turcicus sp. nov., a catalase-negative new member of the Helicobacter genus, isolated from Anatolian Ground Squirrel (Spermophilus xanthoprymnus) in Turkey.
Aydin, Fuat; Karakaya, Emre; Kayman, Tuba; Abay, Secil; Saticioglu, Izzet Burcin.
Afiliación
  • Aydin F; Department of Microbiology, Faculty of Veterinary Medicine, Erciyes University, 38280, Kayseri, Turkey.
  • Karakaya E; Department of Microbiology, Faculty of Veterinary Medicine, Erciyes University, 38280, Kayseri, Turkey.
  • Kayman T; Medical Microbiology Clinic, Sisli Hamidiye Etfal Training and Research Hospital, University of Health Sciences, 34371 Istanbul, Turkey.
  • Abay S; Department of Microbiology, Faculty of Veterinary Medicine, Erciyes University, 38280, Kayseri, Turkey.
  • Saticioglu IB; Bursa Uludag University, Vocational School of Keles, 16740, Bursa, Turkey.
Int J Syst Evol Microbiol ; 72(5)2022 May.
Article en En | MEDLINE | ID: mdl-35506980
Eleven Gram-negative, curved and S-shaped, oxidase activity positive, catalase activity negative bacterial isolates recovered from faeces of Anatolian ground squirrel (Spermophilus xanthoprymnus) in the city of Kayseri, Turkey, were subjected to a polyphasic taxonomic study. Results of a genus-specific PCR revealed that these isolates belonged to the genus Helicobacter. The 16S rRNA gene sequence analysis revealed that the 11 isolates had over 99 % sequence identity with each other and were most closely related to Helicobacter ganmani CMRI H02T with 97.0-97.1 % identity levels and they formed a novel phylogenetic line within the genus Helicobacter. Faydin-H64 and Faydin-H70T strains were subjected to gyrA and atpA gene and whole genome sequence analyses. These two Helicobacter strains formed separate phylogenetic clades, divergent from other known Helicobacter species. The DNA G+C content and genome size of the strain Faydin-H70T were 35.3 mol% and 1.7 Mb, respectively. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain Faydin-H70T and its close phylogenetic neighbour H. winghamensis ATCC BAA-430T were determined as 81.7 and 34.9 %, respectively. Pairwise sequence comparison showed that it was closely related to H. ganmani CMRI H02T however it shared the highest ANI and dDDH values with H. winghamensis ATCC BAA-430T. The data obtained from the polyphasic taxonomy approach, including phenotypic characterization and whole-genome sequences, revealed that these strains represent a novel species within the genus Helicobacter, for which the name Helicobacter turcicus sp. nov., is proposed with Faydin-H70T as the type strain (=DSM 112556T=LMG 32335T).
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sciuridae / Helicobacter Límite: Animals País/Región como asunto: Asia Idioma: En Revista: Int J Syst Evol Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sciuridae / Helicobacter Límite: Animals País/Región como asunto: Asia Idioma: En Revista: Int J Syst Evol Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Reino Unido