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OxLDL induces membrane structure rearrangement leading to biomechanics alteration and migration deficiency in macrophage.
Rezende, Luisa; Couto, Natalia Fernanda Do; Fernandes-Braga, Weslley; Epshtein, Yulia; Alvarez-Leite, Jacqueline Isaura; Levitan, Irena; Andrade, Luciana de Oliveira.
Afiliación
  • Rezende L; Department of Morphology/Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
  • Couto NFD; Department of Morphology/Federal University of Minas Gerais, Belo Horizonte, MG, Brazil; Department of Medicine, University of Illinois at Chicago, Chicago, USA.
  • Fernandes-Braga W; Department of Biochemistry and Immunology/Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
  • Epshtein Y; Department of Medicine, University of Illinois at Chicago, Chicago, USA.
  • Alvarez-Leite JI; Department of Biochemistry and Immunology/Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
  • Levitan I; Department of Medicine, University of Illinois at Chicago, Chicago, USA.
  • Andrade LO; Department of Morphology/Federal University of Minas Gerais, Belo Horizonte, MG, Brazil. Electronic address: lucianaandrade@ufmg.br.
Biochim Biophys Acta Biomembr ; 1864(9): 183951, 2022 09 01.
Article en En | MEDLINE | ID: mdl-35504320
Cholesterol sequestration from plasma membrane has been shown to induce lipid packing disruption, causing actin cytoskeleton reorganization and polymerization, increasing cell stiffness and inducing lysosomal exocytosis in non-professional phagocytes. Similarly, oxidized form of low-density lipoprotein (oxLDL) has also been shown to disrupt lipid organization and packing in endothelial cells, leading to biomechanics alterations that interfere with membrane injury and repair. For macrophages, much is known about oxLDL effects in cell activation, cytokine production and foam cell formation. However, little is known about its impact in the organization of macrophage membrane structured domains and cellular mechanics, the focus of the present study. Treatment of bone marrow-derived macrophages (BMDM) with oxLDL not only altered membrane structure, and potentially the distribution of raft domains, but also induced actin rearrangement, diffuse integrin distribution and cell shrinkage, similarly to observed upon treatment of these cells with MßCD. Those alterations led to decreased migration efficiency. For both treatments, higher co-localization of actin cytoskeleton and GM1 was observed, indicating a similar mechanism of action involving raft-like domain dynamics. Lastly, like MßCD treatment, oxLDL also induced lysosomal spreading in BMDM. We propose that OxLDL induced re-organization of membrane/cytoskeleton complex in macrophages can be attributed to the insertion of oxysterols into the membrane, which lead to changes in lipid organization and disruption of membrane structure, similar to the effect of cholesterol depletion by MßCD treatment. These results indicate that oxLDL can induce physical alterations in the complex membrane/cytoskeleton of macrophages, leading to significant biomechanical changes that compromise cell behavior.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Endoteliales / Lipoproteínas LDL Idioma: En Revista: Biochim Biophys Acta Biomembr Año: 2022 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Endoteliales / Lipoproteínas LDL Idioma: En Revista: Biochim Biophys Acta Biomembr Año: 2022 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Países Bajos