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Virulence strategies of an insect herbivore and oomycete plant pathogen converge on host E3 SUMO ligase SIZ1.
Liu, Shan; Lenoir, Camille J G; Amaro, Tiago M M M; Rodriguez, Patricia A; Huitema, Edgar; Bos, Jorunn I B.
Afiliación
  • Liu S; Division of Plant Sciences, School of Life Sciences, University of Dundee, Dundee, DD2 5DA, UK.
  • Lenoir CJG; Division of Plant Sciences, School of Life Sciences, University of Dundee, Dundee, DD2 5DA, UK.
  • Amaro TMMM; Cell and Molecular Sciences, The James Hutton Institute, Invergowrie, Dundee, DD2 5DA, UK.
  • Rodriguez PA; Division of Plant Sciences, School of Life Sciences, University of Dundee, Dundee, DD2 5DA, UK.
  • Huitema E; Cell and Molecular Sciences, The James Hutton Institute, Invergowrie, Dundee, DD2 5DA, UK.
  • Bos JIB; Division of Plant Sciences, School of Life Sciences, University of Dundee, Dundee, DD2 5DA, UK.
New Phytol ; 235(4): 1599-1614, 2022 08.
Article en En | MEDLINE | ID: mdl-35491752
Pathogens and pests secrete proteins (effectors) to interfere with plant immunity through modification of host target functions and disruption of immune signalling networks. The extent of convergence between pathogen and herbivorous insect virulence strategies is largely unexplored. We found that effectors from the oomycete pathogen, Phytophthora capsici, and the major aphid pest, Myzus persicae target the host immune regulator SIZ1, an E3 SUMO ligase. We used transient expression assays in Nicotiana benthamiana as well as Arabidopsis mutants to further characterize biological role of effector-SIZ1 interactions in planta. We show that the oomycete and aphid effector, which both contribute to virulence, feature different activities towards SIZ1. While M. persicae effector Mp64 increases SIZ1 protein levels in transient assays, P. capsici effector CRN83_152 enhances SIZ1-E3 SUMO ligase activity in vivo. SIZ1 contributes to host susceptibility to aphids and an oomycete pathogen. Knockout of SIZ1 in Arabidopsis decreased susceptibility to aphids, independent of SNC1, PAD4 and EDS1. Similarly SIZ1 knockdown in N. benthamiana led to reduced P. capsici infection. Our results suggest convergence of distinct pathogen and pest virulence strategies on an E3 SUMO ligase to enhance host susceptibility.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Áfidos / Phytophthora / Arabidopsis / Proteínas de Arabidopsis Límite: Animals Idioma: En Revista: New Phytol Asunto de la revista: BOTANICA Año: 2022 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Áfidos / Phytophthora / Arabidopsis / Proteínas de Arabidopsis Límite: Animals Idioma: En Revista: New Phytol Asunto de la revista: BOTANICA Año: 2022 Tipo del documento: Article Pais de publicación: Reino Unido