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Prebiotic effect of sorghum biomass xylooligosaccharides employing immobilized endoxylanase from Thermomyces lanuginosus PC7S1T.
Nascimento, Andreza Gambelli Lucas Costa; Zuppa, Érica Sabrina; Zonin, Maiara; de Sousa, Gabriela Furlaneto Sanchez; de Andrades, Diandra; Ayub, Marco Antônio Záchia; Maller, Alexandre; da Conceição Silva, José Luis; de Cássia Garcia Simão, Rita; Kadowaki, Marina Kimiko.
Afiliación
  • Nascimento AGLC; Center of Medical Sciences and Pharmaceutical, Western Paraná State University, Rua Universitária, 2069, Cascavel, PR, ZC 85814-110, Brazil.
  • Zuppa ÉS; Center of Medical Sciences and Pharmaceutical, Western Paraná State University, Rua Universitária, 2069, Cascavel, PR, ZC 85814-110, Brazil.
  • Zonin M; Center of Medical Sciences and Pharmaceutical, Western Paraná State University, Rua Universitária, 2069, Cascavel, PR, ZC 85814-110, Brazil.
  • de Sousa GFS; Center of Medical Sciences and Pharmaceutical, Western Paraná State University, Rua Universitária, 2069, Cascavel, PR, ZC 85814-110, Brazil.
  • de Andrades D; Biotechnology Bioprocess and Biocatalysis Group, Food Science and Technology Institute, Federal University of Rio Grande Do Sul, Av. Bento Gonçalves 9500, PO Box 15090, Porto Alegre, RS, ZC 91501-970, Brazil.
  • Ayub MAZ; Biotechnology Bioprocess and Biocatalysis Group, Food Science and Technology Institute, Federal University of Rio Grande Do Sul, Av. Bento Gonçalves 9500, PO Box 15090, Porto Alegre, RS, ZC 91501-970, Brazil.
  • Maller A; Center of Medical Sciences and Pharmaceutical, Western Paraná State University, Rua Universitária, 2069, Cascavel, PR, ZC 85814-110, Brazil.
  • da Conceição Silva JL; Center of Medical Sciences and Pharmaceutical, Western Paraná State University, Rua Universitária, 2069, Cascavel, PR, ZC 85814-110, Brazil.
  • de Cássia Garcia Simão R; Center of Medical Sciences and Pharmaceutical, Western Paraná State University, Rua Universitária, 2069, Cascavel, PR, ZC 85814-110, Brazil.
  • Kadowaki MK; Center of Medical Sciences and Pharmaceutical, Western Paraná State University, Rua Universitária, 2069, Cascavel, PR, ZC 85814-110, Brazil. marinakk@gmail.com.
Braz J Microbiol ; 53(3): 1167-1174, 2022 Sep.
Article en En | MEDLINE | ID: mdl-35482283
Purified endoxylanase from Thermomyces lanuginosus PC7S1T was immobilized in calcium alginate, resulting in a yield of 78.5% and a reusability for 11 cycles. The stability of the immobilized enzyme was given for a pH range of 4 to 9 for 96 h. Endoxylanase immobilized in calcium alginate at 65 °C exhibited thermal stability equal to the soluble enzyme for 5 h, and at high temperatures of 75 °C and 85 °C showed half-lives of 4 and 3 h, respectively. Both soluble endoxylanase and immobilized forms were able to hydrolyze hemicellulose, obtained from low-lignin sorghum biomass pretreated with 5% H2O2 and 2% NaOH, after 1 h of incubation at 65 °C, releasing a mixture of short-chain xylooligosaccharides (X2-X6). The highest amounts of XOS generated were those for X5 (24 to 40%), X4 (33 to 39%), and X3 (11 to 22%). These XOS acted as prebiotics, promoting the growth of the probiotic L. acidophilus, similar to glucose in the MRS broth. These results show the potential of low-lignin sorghum to generate XOS with prebiotic activity, suggesting the application of these compounds in the food industry.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sorghum / Endo-1,4-beta Xilanasas Idioma: En Revista: Braz J Microbiol Año: 2022 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil
Texto completo
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Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sorghum / Endo-1,4-beta Xilanasas Idioma: En Revista: Braz J Microbiol Año: 2022 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil