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Depletion of miR-96 Delays, But Does Not Arrest, Photoreceptor Development in Mice.
Xiang, Lue; Zhang, Juan; Rao, Feng-Qin; Yang, Qiao-Li; Zeng, Hui-Yi; Huang, Sheng-Hai; Xie, Zhen-Xiang; Lv, Ji-Neng; Lin, Dan; Chen, Xue-Jiao; Wu, Kun-Chao; Lu, Fan; Huang, Xiu-Feng; Chen, Qi.
Afiliación
  • Xiang L; School of Ophthalmology and Optometry, School of Biomedical Engineering, Eye Hospital, Wenzhou Medical University, Wenzhou, China.
  • Zhang J; State Key Laboratory of Ophthalmology, Optometry and Vision Science, Wenzhou, China.
  • Rao FQ; School of Ophthalmology and Optometry, School of Biomedical Engineering, Eye Hospital, Wenzhou Medical University, Wenzhou, China.
  • Yang QL; School of Ophthalmology and Optometry, School of Biomedical Engineering, Eye Hospital, Wenzhou Medical University, Wenzhou, China.
  • Zeng HY; School of Pharmaceutical Sciences of Wenzhou Medical University, Wenzhou, China.
  • Huang SH; School of Ophthalmology and Optometry, School of Biomedical Engineering, Eye Hospital, Wenzhou Medical University, Wenzhou, China.
  • Xie ZX; School of Ophthalmology and Optometry, School of Biomedical Engineering, Eye Hospital, Wenzhou Medical University, Wenzhou, China.
  • Lv JN; School of Ophthalmology and Optometry, School of Biomedical Engineering, Eye Hospital, Wenzhou Medical University, Wenzhou, China.
  • Lin D; School of Ophthalmology and Optometry, School of Biomedical Engineering, Eye Hospital, Wenzhou Medical University, Wenzhou, China.
  • Chen XJ; School of Ophthalmology and Optometry, School of Biomedical Engineering, Eye Hospital, Wenzhou Medical University, Wenzhou, China.
  • Wu KC; State Key Laboratory of Ophthalmology, Optometry and Vision Science, Wenzhou, China.
  • Lu F; School of Ophthalmology and Optometry, School of Biomedical Engineering, Eye Hospital, Wenzhou Medical University, Wenzhou, China.
  • Huang XF; School of Ophthalmology and Optometry, School of Biomedical Engineering, Eye Hospital, Wenzhou Medical University, Wenzhou, China.
  • Chen Q; State Key Laboratory of Ophthalmology, Optometry and Vision Science, Wenzhou, China.
Invest Ophthalmol Vis Sci ; 63(4): 24, 2022 04 01.
Article en En | MEDLINE | ID: mdl-35481839
Purpose: Abundant retinal microRNA-183 cluster (miR-183C) has been reported to be a key player in photoreceptor development and functionality in mice. However, whether there is a protagonist in this cluster remains unclear. Here, we used a mutant mouse model to study the role of miR-96, a member of miR-183C, in photoreceptor development and functionality. Methods: The mature miR-96 sequence was removed using the CRISPR/Cas9 genome-editing system. Electroretinogram (ERG) and optical coherence tomography (OCT) investigated the changes in structure and function in mouse retinas. Immunostaining determined the localization and morphology of the retinal cells. RNA sequencing was conducted to observe retinal transcription alterations. Results: The miR-96 mutant mice exhibited cone developmental delay, as occurs in miR-183/96 double knockout mice. Immunostaining of cone-specific marker genes revealed cone nucleus mislocalization and exiguous Opn1mw/Opn1sw in the mutant (MT) mouse outer segments at postnatal day 10. Interestingly, this phenomenon could be relieved in the adult stages. Transcriptome analysis revealed activation of microtubule-, actin filament-, and cilia-related pathways, further supporting the findings. Based on ERG and OCT results at different ages, the MT mice displayed developmental delay not only in cones but also in rods. In addition, a group of miR-96 potential direct and indirect target genes was summarized for interpretation and further studies of miR-96-related retinal developmental defects. Conclusions: Depletion of miR-96 delayed but did not arrest photoreceptor development in mice. This miRNA is indispensable for mouse photoreceptor maturation, especially for cones.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Fotorreceptoras Retinianas Conos / MicroARNs Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Invest Ophthalmol Vis Sci Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Fotorreceptoras Retinianas Conos / MicroARNs Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Invest Ophthalmol Vis Sci Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos