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Storage of red blood cells in alkaline PAGGGM improves metabolism but has no effect on recovery after transfusion.
de Bruin, Sanne; Peters, Anna-Linda; Wijnberge, Marije; van Baarle, Floor E H P; AbdelRahman, Amira H A; Vermeulen, Christie; Beuger, Boukje M; Reisz, Julie A; D'Alessandro, Angelo; Vlaar, Alexander P J; de Korte, Dirk; van Bruggen, Robin.
Afiliación
  • de Bruin S; Department of Blood Cell Research, Sanquin Research and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
  • Peters AL; Department of Intensive Care Medicine, Amsterdam University Medical Centers, University of Amsterdam, Amsterdam, The Netherlands.
  • Wijnberge M; Laboratory of Experimental Intensive Care and Anesthesia, Amsterdam University Medical Centers, location AMC, Amsterdam, The Netherlands.
  • van Baarle FEHP; Department of Intensive Care Medicine, Amsterdam University Medical Centers, University of Amsterdam, Amsterdam, The Netherlands.
  • AbdelRahman AHA; Department of Anesthesiology, University Medical Center Utrecht, Utrecht, The Netherlands.
  • Vermeulen C; Laboratory of Experimental Intensive Care and Anesthesia, Amsterdam University Medical Centers, location AMC, Amsterdam, The Netherlands.
  • Beuger BM; Department of Anesthesiology, Amsterdam University Medical Centers, University of Amsterdam, Amsterdam, The Netherlands.
  • Reisz JA; Department of Intensive Care Medicine, Amsterdam University Medical Centers, University of Amsterdam, Amsterdam, The Netherlands.
  • D'Alessandro A; Laboratory of Experimental Intensive Care and Anesthesia, Amsterdam University Medical Centers, location AMC, Amsterdam, The Netherlands.
  • Vlaar APJ; Department of Intensive Care Medicine, Amsterdam University Medical Centers, University of Amsterdam, Amsterdam, The Netherlands.
  • de Korte D; Laboratory of Experimental Intensive Care and Anesthesia, Amsterdam University Medical Centers, location AMC, Amsterdam, The Netherlands.
  • van Bruggen R; Department of Clinical Pathology, Suez Canal University, Ismailia, Egypt.
Blood Adv ; 6(13): 3899-3910, 2022 07 12.
Article en En | MEDLINE | ID: mdl-35477178
Additive solutions are used to limit changes that red blood cells (RBCs) undergo during storage. Several studies have shown better preservation of glucose and redox metabolism using the alkaline additive solution PAGGGM (phosphate-adenine-glucose-guanosine-gluconate-mannitol). In this randomized open-label intervention trial in 20 healthy volunteers, the effect of storage, PAGGGM vs SAGM (saline-adenine-glucose-mannitol), on posttransfusion recovery (PTR) and metabolic restoration after transfusion was assessed. Subjects received an autologous biotinylated RBC concentrate stored for 35 days in SAGM or PAGGGM. As a reference for the PTR, a 2-day stored autologous biotinylated RBC concentrate stored in SAGM was simultaneously transfused. RBC phenotype and PTR were assessed after transfusion. Biotinylated RBCs were isolated from the circulation for metabolomics analysis up to 24 hours after transfusion. The PTR was significantly higher in the 2-day stored RBCs than in 35-day stored RBCs 2 and 7 days after transfusion: 96% (90 to 99) vs 72% (66 to 89) and 96% (90 to 99) vs 72% (66 to 89), respectively. PTR of SAGM- and PAGGGM-stored RBCs did not differ significantly. Glucose and redox metabolism were better preserved in PAGGGM-stored RBCs. The differences measured in the blood bag remained present only until 1 day after transfusion. No differences in RBC phenotype were found besides an increased complement C3 deposition on 35-day RBCs stored in PAGGGM. Our data indicate that despite better metabolic preservation, PAGGGM is not a suitable alternative for SAGM because storage in PAGGGM did not result in an increased PTR. Finally, RBCs recovered from circulation after transfusion showed reversal of the metabolic storage lesion in vivo within a day. This study is registered in the Dutch trial register (NTR6492).
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Conservación de la Sangre / Adenina Tipo de estudio: Clinical_trials Límite: Humans Idioma: En Revista: Blood Adv Año: 2022 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Estados Unidos
Texto completo
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Imprimir
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Conservación de la Sangre / Adenina Tipo de estudio: Clinical_trials Límite: Humans Idioma: En Revista: Blood Adv Año: 2022 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Estados Unidos