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Effects of silencing neuropilin-2 on proliferation, migration, and invasion of colorectal cancer HT-29.
Zhan, Qi-Qi; Liu, Qian-Yu; Yang, Xin; Ge, Yu-Hui; Xu, Lei; Ding, Guan-Yi; Guo, Shang; Zhu, Bing; Xu, Wei-Guo.
Afiliación
  • Zhan QQ; Department of Surgical Oncology, North China University of Science and Technology Affiliated Hospital, Tangshan, Hebei, China.
  • Liu QY; Department of General Surgery, North China University of Science and Technology Affiliated Hospital, Tangshan, Hebei, China.
  • Yang X; Department of Surgical Oncology, North China University of Science and Technology Affiliated Hospital, Tangshan, Hebei, China.
  • Ge YH; Department of Surgical Oncology, North China University of Science and Technology Affiliated Hospital, Tangshan, Hebei, China.
  • Xu L; Department of Surgical Oncology, North China University of Science and Technology Affiliated Hospital, Tangshan, Hebei, China.
  • Ding GY; Department of Surgical Oncology, North China University of Science and Technology Affiliated Hospital, Tangshan, Hebei, China.
  • Guo S; Department of Surgical Oncology, North China University of Science and Technology Affiliated Hospital, Tangshan, Hebei, China.
  • Zhu B; Department of Surgical Oncology, North China University of Science and Technology Affiliated Hospital, Tangshan, Hebei, China.
  • Xu WG; Department of Surgical Oncology, North China University of Science and Technology Affiliated Hospital, Tangshan, Hebei, China.
Bioengineered ; 13(4): 11042-11049, 2022 04.
Article en En | MEDLINE | ID: mdl-35473507
To investigate the effects of silencing neuropilin-2(NRP-2) on the proliferation, migration, and invasion of colorectal cancer(CRC) HT-29. Lipofectamine 2000 was used to transfect specific siRNA for NRP-2 and nonspecific control siRNA into human colorectal cancer HT-29 as the transfection group and meaningless sequence group. HT-29 cultured in a medium was used as the blank control group. The expression levels of NRP-2 mRNA in the cells were detected by real-time fluorescence quantitative PCR. The expressions of proliferation-associated protein Ki-67 in the cells were detected by immunochemical staining. Migration ability was assessed by a monolayer cell scratch wound damage and repair experiment. The Transwell chamber invasion experiment was adopted to determine invasive ability by measuring the number of tumor cells crossing the chamber membrane. Compared with the meaningless sequence group and blank control group, real-time fluorescence quantitative PCR showed that the relative expression level of NRP-2 mRNA in the transfection group was significantly decreased(P < 0.05). Results of immunochemical staining revealed that the expression of Ki-67 protein in the transfected cells was significantly reduced, and the proliferation ability was decreased(P < 0.05). The results further showed that the scratch healing rate of the transfected cells decreased after 24 h of healing(P < 0.05). Results of Transwell invasion assay showed that the number of cells passing through the stromal membrane of the upper chamber to the back of the chamber was significantly reduced in the transfection group(p < 0.05). Silencing NRP-2 could inhibit the proliferation, migration, and invasion of colorectal cancer HT-29.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Colorrectales / Neuropilina-2 Límite: Humans Idioma: En Revista: Bioengineered Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Colorrectales / Neuropilina-2 Límite: Humans Idioma: En Revista: Bioengineered Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos