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An applicable method for extraction of whole seeds protein and its determination through Bradford's method.
Karimi, Fatemeh; Hamidian, Yasamin; Behrouzifar, Fatemeh; Mostafazadeh, Reza; Ghorbani-HasanSaraei, Azade; Alizadeh, Marzieh; Mortazavi, Seyed-Morteza; Janbazi, Mobina; Naderi Asrami, Padideh.
Afiliación
  • Karimi F; Department of Chemical Engineering, Quchan University of Technology, Quchan, Iran. Electronic address: fkm024@gmail.com.
  • Hamidian Y; Department of Chemical Engineering, Quchan University of Technology, Quchan, Iran. Electronic address: yasiii1992@gmail.com.
  • Behrouzifar F; Skills and Entrepreneurship College, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran; Nikan Salamat Kemend Khazar, Sari, Iran. Electronic address: f.behrouzifar@gmail.com.
  • Mostafazadeh R; Department of Chemistry, Iran University of Science and Technology (IUST), Tehran, Iran.
  • Ghorbani-HasanSaraei A; Department of Food Science and Technology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran. Electronic address: azade380@yahoo.com.
  • Alizadeh M; Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Laboratory of Basic Sciences, Mohammad Rasul Allah Research Tower, Shiraz University of Medical Sciences, Shiraz, 71348-14336, Iran. Electronic address: m_alizadeh64@yahoo.com.
  • Mortazavi SM; Department of Food Science and Technology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran. Electronic address: s.morteza.mortazavai@gmail.com.
  • Janbazi M; Department of Chemistry, Golestan University, Gorgan, Iran.
  • Naderi Asrami P; Department of Chemistry, Massoud Clinical Laboratory, Tehran, Iran. Electronic address: p.naderi@gmail.com.
Food Chem Toxicol ; 164: 113053, 2022 Jun.
Article en En | MEDLINE | ID: mdl-35460823
Analysis of protein content of food is necessary for quality control and is essential for precise labeling. Protein analysis is an issue of great economic and social fondness. Cereals are one of the most important sources of protein in food, livestock and poultry feed. In this article, the technique of extracting protein in 4 types of grains and measuring it by the Bradford method is discussed. The results obtained from this method are compared with the data obtained by the Kjeldahl method. This comparison showed that the Bradford method is more accurate in measuring proteins. Extraction of protein using NaOH at pH 13 can be used as a modified method to release proteins in soybean meal and consequently a Fast and accurate high-performance laboratory determination method for protein content via the Bradford method. The optimum pH value was identified as that of 13 in optimum temperature 40 °C for maximum protein extraction yield (43.6%, w/w). The new method used in this paper has resulted in the measurement of grain protein in the shortest time and with the least toxicity and the highest accuracy.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Semillas / Glycine max Idioma: En Revista: Food Chem Toxicol Año: 2022 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Semillas / Glycine max Idioma: En Revista: Food Chem Toxicol Año: 2022 Tipo del documento: Article Pais de publicación: Reino Unido