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Aflatoxin B1 DNA-Adducts in Hepatocellular Carcinoma from a Low Exposure Area.
Gramantieri, Laura; Gnudi, Federica; Vasuri, Francesco; Mandrioli, Daniele; Fornari, Francesca; Tovoli, Francesco; Suzzi, Fabrizia; Vornoli, Andrea; D'Errico, Antonia; Piscaglia, Fabio; Giovannini, Catia.
Afiliación
  • Gramantieri L; Division of Internal Medicine, Hepatobiliary and Immunoallergic Diseases, IRCCS Azienda Ospedaliero-Universitaria di Bologna, 40138 Bologna, Italy.
  • Gnudi F; Cesare Maltoni Cancer Research Center, Ramazzini Institute, Via Saliceto 3, 40010 Bentivoglio, Italy.
  • Vasuri F; Pathology Unit, IRCCS Azienda Ospedaliero-Universitaria di Bologna, 40138 Bologna, Italy.
  • Mandrioli D; Cesare Maltoni Cancer Research Center, Ramazzini Institute, Via Saliceto 3, 40010 Bentivoglio, Italy.
  • Fornari F; Department for Life Quality Studies, University of Bologna, 40126 Rimini, Italy.
  • Tovoli F; Center for Applied Biomedical Research (CRBA), University of Bologna, 40138 Bologna, Italy.
  • Suzzi F; Division of Internal Medicine, Hepatobiliary and Immunoallergic Diseases, IRCCS Azienda Ospedaliero-Universitaria di Bologna, 40138 Bologna, Italy.
  • Vornoli A; Cesare Maltoni Cancer Research Center, Ramazzini Institute, Via Saliceto 3, 40010 Bentivoglio, Italy.
  • D'Errico A; Center for Applied Biomedical Research (CRBA), University of Bologna, 40138 Bologna, Italy.
  • Piscaglia F; Cesare Maltoni Cancer Research Center, Ramazzini Institute, Via Saliceto 3, 40010 Bentivoglio, Italy.
  • Giovannini C; Pathology Unit, IRCCS Azienda Ospedaliero-Universitaria di Bologna, 40138 Bologna, Italy.
Nutrients ; 14(8)2022 Apr 15.
Article en En | MEDLINE | ID: mdl-35458213
Aflatoxin B1 (AFB1) is a class 1 carcinogen with an ascertained role in the development of hepatocellular carcinoma (HCC) in high exposure areas. Instead, this study aimed to assay whether chronic/intermittent, low-dose AFB1 consumption might occur in low-exposure geographical areas, ultimately accumulating in the liver and possibly contributing to liver cancer. AFB1-DNA adducts were assayed by immunostaining in liver tissues from three Italian series of twenty cirrhosis without HCC, 131 HCC, and 45 cholangiocarcinoma, and in an AFB1-induced HCC rat model. CD68, TP53 immunostaining, and TP53 RFLP analysis of R249S transversion were used to characterize cell populations displaying AFB1-DNA adducts. Twenty-five HCCs displayed AFB1-adducts both in neoplastic hepatocytes and in cells infiltrating the tumor and non-tumor tissues. Nuclear immunostaining was observed in a few cases, while most cases showed cytoplasmic immunostaining, especially in CD68-positive tumor-infiltrating cells, suggestive for phagocytosis of dead hepatocytes. Similar patterns were observed in AFB1-induced rat HCC, though with higher intensity. Cholangiocarcinoma and cirrhosis without HCC did not displayAFB1-adducts, except for one case. Despite not providing a causal relationship with HCC, these findings still suggest paying attention to detection and control measures for aflatoxins to ensure food safety in low exposure areas.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Colangiocarcinoma / Carcinoma Hepatocelular / Neoplasias Hepáticas Tipo de estudio: Etiology_studies / Prognostic_studies Límite: Animals Idioma: En Revista: Nutrients Año: 2022 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Colangiocarcinoma / Carcinoma Hepatocelular / Neoplasias Hepáticas Tipo de estudio: Etiology_studies / Prognostic_studies Límite: Animals Idioma: En Revista: Nutrients Año: 2022 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza