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Transcriptional Profiling of the Small Intestine and the Colon Reveals Modulation of Gut Infection with Citrobacter rodentium According to the Vitamin A Status.
Chai, Zhi; Lyu, Yafei; Chen, Qiuyan; Wei, Cheng-Hsin; Snyder, Lindsay M; Weaver, Veronika; Sebastian, Aswathy; Albert, István; Li, Qunhua; Cantorna, Margherita T; Ross, Catharine.
Afiliación
  • Chai Z; Intercollege Graduate Degree Program in Physiology, The Pennsylvania State University, University Park, PA 16802, USA.
  • Lyu Y; Department of Nutritional Sciences, The Pennsylvania State University, University Park, PA 16802, USA.
  • Chen Q; Intercollege Graduate Degree Program in Bioinformatics and Genomics, The Pennsylvania State University, University Park, PA 16802, USA.
  • Wei CH; Department of Nutritional Sciences, The Pennsylvania State University, University Park, PA 16802, USA.
  • Snyder LM; Department of Nutritional Sciences, The Pennsylvania State University, University Park, PA 16802, USA.
  • Weaver V; Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802, USA.
  • Sebastian A; Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802, USA.
  • Albert I; Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA 16802, USA.
  • Li Q; Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA.
  • Cantorna MT; Department of Statistics, The Pennsylvania State University, University Park, PA 16802, USA.
  • Ross C; Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802, USA.
Nutrients ; 14(8)2022 Apr 08.
Article en En | MEDLINE | ID: mdl-35458125
Vitamin A (VA) deficiency and diarrheal diseases are both serious public health issues worldwide. VA deficiency is associated with impaired intestinal barrier function and increased risk of mucosal infection-related mortality. The bioactive form of VA, retinoic acid, is a well-known regulator of mucosal integrity. Using Citrobacter rodentium-infected mice as a model for diarrheal diseases in humans, previous studies showed that VA-deficient (VAD) mice failed to clear C. rodentium as compared to their VA-sufficient (VAS) counterparts. However, the distinct intestinal gene responses that are dependent on the host's VA status still need to be discovered. The mRNAs extracted from the small intestine (SI) and the colon were sequenced and analyzed on three levels: differential gene expression, enrichment, and co-expression. C. rodentium infection interacted differentially with VA status to alter colon gene expression. Novel functional categories downregulated by this pathogen were identified, highlighted by genes related to the metabolism of VA, vitamin D, and ion transport, including improper upregulation of Cl- secretion and disrupted HCO3- metabolism. Our results suggest that derangement of micronutrient metabolism and ion transport, together with the compromised immune responses in VAD hosts, may be responsible for the higher mortality to C. rodentium under conditions of inadequate VA.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Deficiencia de Vitamina A / Infecciones por Enterobacteriaceae Límite: Animals Idioma: En Revista: Nutrients Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Deficiencia de Vitamina A / Infecciones por Enterobacteriaceae Límite: Animals Idioma: En Revista: Nutrients Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Suiza