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Genome Characterisation of an Isoprene-Degrading Alcaligenes sp. Isolated from a Tropical Restored Forest.
Uttarotai, Toungporn; Sutheeworapong, Sawannee; Crombie, Andrew T; Murrell, J Colin; Mhuantong, Wuttichai; Noirungsee, Nuttapol; Wangkarn, Sunanta; Bovonsombut, Sakunnee; McGenity, Terry J; Chitov, Thararat.
Afiliación
  • Uttarotai T; Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
  • Sutheeworapong S; School of Life Sciences, University of Essex, Colchester CO4 3SQ, UK.
  • Crombie AT; School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkok 10150, Thailand.
  • Murrell JC; School of Environmental Sciences, University of East Anglia, Norwich NR4 7TJ, UK.
  • Mhuantong W; School of Environmental Sciences, University of East Anglia, Norwich NR4 7TJ, UK.
  • Noirungsee N; Enzyme Technology Research Team, Biorefinery and Bioproduct Technology Research Group, National Center for Genetic Engineering and Biotechnology, Pathumthani 12120, Thailand.
  • Wangkarn S; Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
  • Bovonsombut S; Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
  • McGenity TJ; Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
  • Chitov T; Environmental Science Research Center (ESRC), Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Biology (Basel) ; 11(4)2022 Mar 28.
Article en En | MEDLINE | ID: mdl-35453719
Isoprene is a climate-active biogenic volatile organic compound (BVOC), emitted into the atmosphere in abundance, mainly from terrestrial plants. Soil is an important sink for isoprene due to its consumption by microbes. In this study, we report the ability of a soil bacterium to degrade isoprene. Strain 13f was isolated from soil beneath wild Himalayan cherry trees in a tropical restored forest. Based on phylogenomic analysis and an Average Nucleotide Identity score of >95%, it most probably belongs to the species Alcaligenes faecalis. Isoprene degradation by Alcaligenes sp. strain 13f was measured by using gas chromatography. When isoprene was supplied as the sole carbon and energy source at the concentration of 7.2 × 105 ppbv and 7.2 × 106 ppbv, 32.6% and 19.6% of isoprene was consumed after 18 days, respectively. Genome analysis of Alcaligenes sp. strain 13f revealed that the genes that are typically found as part of the isoprene monooxygenase gene cluster in other isoprene-degrading bacteria were absent. This discovery suggests that there may be alternative pathways for isoprene metabolism.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biology (Basel) Año: 2022 Tipo del documento: Article País de afiliación: Tailandia Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biology (Basel) Año: 2022 Tipo del documento: Article País de afiliación: Tailandia Pais de publicación: Suiza