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Dysregulation of lncRNA-miRNA-mRNA Interactome as a Marker of Metastatic Process in Ovarian Cancer.
Pronina, Irina V; Uroshlev, Leonid A; Moskovtsev, Alexey A; Zaichenko, Danila M; Filippova, Elena A; Fridman, Marina V; Burdennyy, Alexey M; Loginov, Vitaly I; Kazubskaya, Tatiana P; Kushlinskii, Nikolay E; Dmitriev, Alexey A; Braga, Eleonora A; Brovkina, Olga I.
Afiliación
  • Pronina IV; Institute of General Pathology and Pathophysiology, 125315 Moscow, Russia.
  • Uroshlev LA; Institute of General Pathology and Pathophysiology, 125315 Moscow, Russia.
  • Moskovtsev AA; Vavilov Institute of General Genetics, Russian Academy of Sciences, 119991 Moscow, Russia.
  • Zaichenko DM; Institute of General Pathology and Pathophysiology, 125315 Moscow, Russia.
  • Filippova EA; Pirogov Russian National Research Medical University, 117997 Moscow, Russia.
  • Fridman MV; Institute of General Pathology and Pathophysiology, 125315 Moscow, Russia.
  • Burdennyy AM; Institute of General Pathology and Pathophysiology, 125315 Moscow, Russia.
  • Loginov VI; Vavilov Institute of General Genetics, Russian Academy of Sciences, 119991 Moscow, Russia.
  • Kazubskaya TP; Institute of General Pathology and Pathophysiology, 125315 Moscow, Russia.
  • Kushlinskii NE; Institute of General Pathology and Pathophysiology, 125315 Moscow, Russia.
  • Dmitriev AA; N. N. Blokhin National Medical Research Center of Oncology, 115478 Moscow, Russia.
  • Braga EA; N. N. Blokhin National Medical Research Center of Oncology, 115478 Moscow, Russia.
  • Brovkina OI; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia.
Biomedicines ; 10(4)2022 Mar 31.
Article en En | MEDLINE | ID: mdl-35453574
Ovarian cancer (OC) is one of the most common types of cancer among malignancies of the female reproductive system. This pathology is asymptomatic until advanced stages and has a poor prognosis. Our study aimed to search for lncRNA-miRNA-mRNA competing triplets that promote ovarian tumorigenesis. For this purpose, we analyzed tumor samples from the TCGA database and verified the results experimentally in a set of 46 paired samples of tumor and matched histologically unchanged ovarian tissues from OC patients. The list of RNAs selected in silico for experimental studies included 13 mRNAs, 10 lncRNAs, and 5 miRNAs related to epithelial-mesenchymal transition and angiogenesis. We evaluated the expression of these RNAs by qRT-PCR and assessed the correlation between levels of miRNAs, mRNAs, and lncRNAs. Sixteen significant triplets were revealed, in some of which, e.g., OIP5-AS1-miR-203a-c-MET and OIP5-AS1-miR-203a-ZEB2, both lncRNA and mRNA had sites for miR-203a direct binding. Transfection of the OVCAR-3 and SKOV-3 cell lines with the miR-203a mimic was used to confirm the novel links of miR-203a with ZEB2 and c-MET in OC. These connections suggest that the interactomes have the potential for diagnostics of metastasis at early onset.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biomedicines Año: 2022 Tipo del documento: Article País de afiliación: Rusia Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biomedicines Año: 2022 Tipo del documento: Article País de afiliación: Rusia Pais de publicación: Suiza