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A functional screen of RNA binding proteins identifies genes that promote or limit the accumulation of CD138+ plasma cells.
Turner, David J; Saveliev, Alexander; Salerno, Fiamma; Matheson, Louise S; Screen, Michael; Lawson, Hannah; Wotherspoon, David; Kranc, Kamil R; Turner, Martin.
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  • Turner DJ; Immunology Programme, The Babraham Institute,Babraham Research Campus, Cambridge, United Kingdom.
  • Saveliev A; RNA Biology Laboratory, Center for Cancer Research, National Cancer Institute (NCI), Frederick, United States.
  • Salerno F; Immunology Programme, The Babraham Institute,Babraham Research Campus, Cambridge, United Kingdom.
  • Matheson LS; Immunology Programme, The Babraham Institute,Babraham Research Campus, Cambridge, United Kingdom.
  • Screen M; Immunology Programme, The Babraham Institute,Babraham Research Campus, Cambridge, United Kingdom.
  • Lawson H; Immunology Programme, The Babraham Institute,Babraham Research Campus, Cambridge, United Kingdom.
  • Wotherspoon D; Laboratory of Haematopoietic Stem Cell and Leukaemia Biology, Queen Mary University of London, London, United Kingdom.
  • Kranc KR; Laboratory of Haematopoietic Stem Cell and Leukaemia Biology, Queen Mary University of London, London, United Kingdom.
  • Turner M; Laboratory of Haematopoietic Stem Cell and Leukaemia Biology, Queen Mary University of London, London, United Kingdom.
Elife ; 112022 04 22.
Article en En | MEDLINE | ID: mdl-35451955
To identify roles of RNA binding proteins (RBPs) in the differentiation or survival of antibody secreting plasma cells we performed a CRISPR/Cas9 knockout screen of 1213 mouse RBPs for their ability to affect proliferation and/or survival, and the abundance of differentiated CD138 + cells in vitro. We validated the binding partners CSDE1 and STRAP as well as the m6A binding protein YTHDF2 as promoting the accumulation of CD138 + cells in vitro. We validated the EIF3 subunits EIF3K and EIF3L and components of the CCR4-NOT complex as inhibitors of CD138 + cell accumulation in vitro. In chimeric mouse models YTHDF2-deficient plasma cells failed to accumulate.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Plasmáticas / Proteínas de Unión al ARN Límite: Animals Idioma: En Revista: Elife Año: 2022 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Plasmáticas / Proteínas de Unión al ARN Límite: Animals Idioma: En Revista: Elife Año: 2022 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Reino Unido