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Effect of platelet-rich plasma (PRP) on post-thaw quality, kinematics and in vivo fertility of fertile and subfertile buffalo (Bubalus bubalis) spermatozoa.
Almadaly, Essam A; Ibrahim, Ibrahim M; Salama, Maha S; Ashour, Mohey A; Sahwan, Ferial M; El-Kon, Ismail I; Abouzed, Tarek K; El-Domany, Wael B.
Afiliación
  • Almadaly EA; Department of Theriogenology, Faculty of Veterinary Medicine, Kafrelsheikh University, El-Geish Street, Kafrelsheikh, 33516, Egypt. dr_essam_2005@yahoo.com.
  • Ibrahim IM; Sakha Animal Production Station, Agricultural Research Center, Ministry of Agriculture, Kafrelsheikh, 33516, Egypt.
  • Salama MS; Animal Production Research Institute (APRI), Kafrelsheikh branch, Ministry of Agriculture, Dokki, Egypt.
  • Ashour MA; Riwina Animal Production Station, Agricultural Research Center, Ministry of Agriculture, Kafrelsheikh, 33516, Egypt.
  • Sahwan FM; Animal Breeding and Production, Animal Husbandry and Animal Wealth Development Department, Faculty of Veterinary Medicine, Alexandria University, Alexandria, Egypt.
  • El-Kon II; Department of Theriogenology, Faculty of Veterinary Medicine, Kafrelsheikh University, El-Geish Street, Kafrelsheikh, 33516, Egypt.
  • Abouzed TK; Department of Biochemistry, Faculty of Veterinary Medicine, Kafrelsheikh University, El-Geish Street, Kafrelsheikh, 33516, Egypt.
  • El-Domany WB; Department of Theriogenology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt.
Vet Res Commun ; 47(1): 61-72, 2023 Jan.
Article en En | MEDLINE | ID: mdl-35451670
This study investigated the effect of adding platelet-rich plasma (PRP) in semen extender prior cryopreservation on post-thaw quality, kinematics, and in vivo fertility of fertile and subfertile buffalo spermatozoa. Eleven buffalo bulls were classified based on their conception rate (CR) into fertile (n = 8, CR > 55%) and subfertile (n = 3, CR < 35%) groups. Ejaculates were collected with artificial vagina, pooled, and dispensed into 6 aliquots, diluted with Tris-egg yolk-glycerol extender supplemented with different proportions of PRP [0% (control), 5%, 10%, 15%, 20%, and 25%] followed by cryopreservation using standard procedures. Post-thaw sperm quality, kinematics, antioxidant activity, cryosurvival rate, and in vivo fertility were compared between fertile and subfertile groups and among proportions of PRP within each group. The results showed that 15% PRP greatly (P < 0.001) improved sperm characteristics, average path velocity, and curvilinear velocity of the subfertile group. Interestingly, 5%, 10%, and 15% PRP greatly (P < 0.001) reduced malondialdehyde content and improved enzymatic (glutathione peroxidase and superoxide dismutase) and total antioxidant capacity in fertile and subfertile groups. However, these three proportions of PRP significantly (P < 0.001) improved the cryosurvival rate of the subfertile group; only 15% PRP greatly improved CR of subfertile (60.83% vs. 34.17%) animals to be comparable with that of fertile ones treated with 5 (59.17%) and 10% (60.83%) PRP. In conclusion, adding 15% PRP to semen extender before cryopreservation is recommended to improve post-thaw quality, antioxidant activity, and in vivo fertility of buffalo semen particularly of the subfertile animals.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Preservación de Semen / Plasma Rico en Plaquetas Límite: Animals Idioma: En Revista: Vet Res Commun Año: 2023 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Preservación de Semen / Plasma Rico en Plaquetas Límite: Animals Idioma: En Revista: Vet Res Commun Año: 2023 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Suiza