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Quantitative serology for SARS-CoV-2 using self-collected saliva and finger-stick blood.
Campbell, Christopher; Padmanabhan, Nikhil; Romero, Daniel; Joe, Jessica; Gebremeskel, Mikias; Manjula, Navaratnam; Wohlstadter, Noah; Wohlstadter, Rachel; Goodwin, Paul; Quintero, Lillian; Debad, Jeff; Sigal, George; Wohlstadter, Jacob.
Afiliación
  • Campbell C; Meso Scale Diagnostics, LLC., 16020 Industrial Drive, Gaithersburg, MD, 20877, USA. ccampbell@mesoscale.com.
  • Padmanabhan N; Meso Scale Diagnostics, LLC., 16020 Industrial Drive, Gaithersburg, MD, 20877, USA.
  • Romero D; Meso Scale Diagnostics, LLC., 16020 Industrial Drive, Gaithersburg, MD, 20877, USA.
  • Joe J; Meso Scale Diagnostics, LLC., 16020 Industrial Drive, Gaithersburg, MD, 20877, USA.
  • Gebremeskel M; Meso Scale Diagnostics, LLC., 16020 Industrial Drive, Gaithersburg, MD, 20877, USA.
  • Manjula N; Meso Scale Diagnostics, LLC., 16020 Industrial Drive, Gaithersburg, MD, 20877, USA.
  • Wohlstadter N; Meso Scale Diagnostics, LLC., 16020 Industrial Drive, Gaithersburg, MD, 20877, USA.
  • Wohlstadter R; Meso Scale Diagnostics, LLC., 16020 Industrial Drive, Gaithersburg, MD, 20877, USA.
  • Goodwin P; Meso Scale Diagnostics, LLC., 16020 Industrial Drive, Gaithersburg, MD, 20877, USA.
  • Quintero L; Meso Scale Diagnostics, LLC., 16020 Industrial Drive, Gaithersburg, MD, 20877, USA.
  • Debad J; Meso Scale Diagnostics, LLC., 16020 Industrial Drive, Gaithersburg, MD, 20877, USA.
  • Sigal G; Meso Scale Diagnostics, LLC., 16020 Industrial Drive, Gaithersburg, MD, 20877, USA.
  • Wohlstadter J; Meso Scale Diagnostics, LLC., 16020 Industrial Drive, Gaithersburg, MD, 20877, USA.
Sci Rep ; 12(1): 6560, 2022 04 21.
Article en En | MEDLINE | ID: mdl-35449177
Convenient and widespread serology testing may alter the trajectory of the COVID-19 pandemic. This study seeks to leverage high-throughput, multiplexed serologic assays, which have been adopted as benchmarks for vaccine efficacy, to support large-scale surveys of SARS-CoV-2 immunity using finger-stick blood and/or saliva. Specifically, we optimized MSD's serology assays, which were analytically validated for serum, to test self-collected finger-stick blood and saliva samples to identify prior infection. We show that these assays can be used with FDA-registered specimen collection devices to obtain quantitative measurements for self-collected samples. First, we show that salivary antibodies are stable without refrigeration or preservatives for at least 5 days. We selected classification thresholds for antibodies against SARS-CoV-2 N, RBD and Spike in finger-stick blood and saliva that provided 98% specificity in a set of individuals without known COVID-19 exposure. Using matched samples, we show that testing of saliva and finger-stick blood equivalently identified individuals with humoral responses to CoV-2 antigens. Moreover, we piloted a simple saliva collection kit that can be used to safely send samples through the mail using written instructions only. This work establishes key parameters to robustly assay self-collected finger-stick blood and saliva using quantitative immunoassays that could support large-scale serology testing.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: SARS-CoV-2 / COVID-19 Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Humans Idioma: En Revista: Sci Rep Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: SARS-CoV-2 / COVID-19 Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Humans Idioma: En Revista: Sci Rep Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido