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Hsa-miR-30a-3p overcomes the acquired protective autophagy of bladder cancer in chemotherapy and suppresses tumor growth and muscle invasion.
Hwang, Thomas I-Sheng; Chen, Po-Chun; Tsai, Te-Fu; Lin, Ji-Fan; Chou, Kuang-Yu; Ho, Chao-Yen; Chen, Hung-En; Chang, An-Chen.
Afiliación
  • Hwang TI; Division of Urology, Department of Surgery, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan.
  • Chen PC; Division of Urology, School of Medicine, Fu-Jen Catholic University, New Taipei, Taiwan.
  • Tsai TF; Department of Urology, Taipei Medical University, Taipei, Taiwan.
  • Lin JF; Department of Life Science, National Taiwan Normal University, Taipei, Taiwan.
  • Chou KY; Translational Medicine Center, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan.
  • Ho CY; Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, Taiwan.
  • Chen HE; Division of Urology, Department of Surgery, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan.
  • Chang AC; Division of Urology, School of Medicine, Fu-Jen Catholic University, New Taipei, Taiwan.
Cell Death Dis ; 13(4): 390, 2022 04 21.
Article en En | MEDLINE | ID: mdl-35449123
Bladder cancer (BC) is the second most common urologic cancer in western countries. New strategies for managing high-grade muscle-invasive bladder cancer (MIBC) are urgently required because MIBC has a high risk of recurrence and poor survival. A growing body of evidence indicates that microRNA has potent antitumorigenic properties in various cancers, and thus, therapeutic strategies based on microRNA may show promising results in cancer therapy. Analysis of The Cancer Genome Atlas (TCGA) database indicated that hsa-miR-30a-3p is downregulated in human BC. Our in vitro investigation demonstrated that hsa-miR-30a-3p suppresses the expression of matrix metalloproteinase-2 (MMP-2) and MMP-9 and reduces the cell invasive potential of BC cells. Furthermore, hsa-miR-30a-3p directly targets ATG5, ATG12, and Beclin 1; this in turn improves the chemosensitivity of BC cells to cisplatin through the repression of protective autophagy. In a tumor-xenograft mice model, hsa-miR-30a-3p suppressed muscle invasion. Cotreatment with hsa-miR-30a-3p enhanced the antitumor effect of cisplatin in reducing tumor growth in BC. The current study provides a novel strategy of using hsa-miR-30a-3p as an adjuvant or replacement therapy in future BC treatment.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Vejiga Urinaria / MicroARNs Límite: Animals / Female / Humans / Male Idioma: En Revista: Cell Death Dis Año: 2022 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Vejiga Urinaria / MicroARNs Límite: Animals / Female / Humans / Male Idioma: En Revista: Cell Death Dis Año: 2022 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Reino Unido